| 孙静,李冰燕,陈跃进,聂继华,童建.氡暴露致小鼠骨髓细胞的DNA损伤[J].中华放射医学与防护杂志,2008,28(5):449-451 |
| 氡暴露致小鼠骨髓细胞的DNA损伤 |
| DNA damage of bone marrow cells in mice induced by radon exposure |
| 投稿时间：2008-05-08 |
| DOI： |
| 中文关键词: 氡 骨髓细胞 DNA损伤 |
| 英文关键词:Radon Bone marrow cells DNA damage |
| 基金项目: |
|
| 摘要点击次数: 2805 |
| 全文下载次数: 1761 |
| 中文摘要: |
| 目的 研究氡暴露致小鼠骨髓细胞的DNA损伤。方法 采用雄性BALB/C小鼠24只,随机分为4组,每组6只,除对照组外,处理组小鼠整体暴露于多功能生态氡室,吸入氡及其子体的累积剂量分别为27(低剂量组)、52(中剂量组)和105(高剂量组)工作水平月(WLM)。采用单细胞凝胶电泳(SCGE)、微核(MN)实验、激光共聚焦显微镜(LSCM)检测小鼠骨髓细胞的DNA链断裂、微核细胞率及凋亡率,观察氡暴露致小鼠骨髓细胞的DNA损伤。结果 高剂量氡暴露可造成小鼠骨髓细胞DNA断裂,尾长和尾DNA百分含量与对照组相比,差异有统计学意义(F=201.9,40.78,P<0.05);微核率和凋亡率增加差异有统计学意义(F=16.28,41.62,P<0.05)。而中、低剂量组则无明显改变。结论 高剂量氡暴露引起DNA损伤,从而对小鼠骨髓细胞产生毒效应。 |
| 英文摘要: |
| Objective To study the DNA damage of bone marrow cells of mice after exposure to Radon. Methods Twenty-four mice were randomly divided into four groups, one control group and three experimental groups with the cumulative doses of radon at 27 WLM(low dose group), 52 WLM(middle dose group) and 105 WLM(high dose group). DNA damage induced by radon in bone marrow of mice was detected by methods of single cell gel electrophoresis (SCGE), micronucleus(MN) and laser scanning confocal microscope (LSCM) observation. Results The DNA strand breakage, rate of MN and apoptosis increased significantly in the high dose group, but not in the middle and low dose groups. Conclusions Exposure to radon could induce DNA damage in bone marrow cells of mice at high levels. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|