| 赵黎,彭瑞云,高亚兵,王水明,王晓民,徐新萍,王丽峰,董霁,杨国山.FDP对微波辐射后大鼠海马COX表达的影响[J].中华放射医学与防护杂志,2008,28(4):421-423 |
| FDP对微波辐射后大鼠海马COX表达的影响 |
| Effects of fructose-1,6-diphosphate on COX expression of mitochondrial respiratory chain in rats hippocampus after microwave exposure |
| 投稿时间：2008-02-28 |
| DOI： |
| 中文关键词: FDP 微波辐射 大鼠 海马 线粒体 COX |
| 英文关键词:FDP Microwave Rat Hippocampus Mitochondria COX |
| 基金项目:军队医药卫生“十一五”专项基金资助项目(06Z065) |
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| 中文摘要: |
| 目的探讨1, 6-二磷酸果糖(FDP)对微波辐射后大鼠海马线粒体呼吸链相关基因细胞色素c氧化酶(cytochrome c oxidase,COX)表达的影响。方法Wistar雄性大鼠随机分为假辐射组(5只),辐射组(10只)和辐射+药物组(10只)。采用30mW/cm2微波辐射,辐射+药物组于辐射前3d 腹腔注射350mg/kg FDP,连续给药3或10d,1次/d。于辐射后6h和7d活杀取海马,采用反转录-聚合酶链反应(RT-PCR)和图像分析检测大鼠海马组织COX亚基Ⅰ、Ⅱ和Ⅳ mRNA表达,通过Western blot和图像分析检测大鼠海马组织COX Ⅰ蛋白表达变化。结果辐射后6h和7d大鼠海马COX Ⅰ、Ⅱ和Ⅳ mRNA表达均较假辐射组降低,药物组大鼠海马COX Ⅰ、Ⅱ和Ⅳ mRNA与相应辐射组相比均见表达增加,以给药后3d(辐射后6h)组增加最为显著(tCOX Ⅰ=3.2205,tCOX Ⅱ=3.5762,tCOX Ⅳ=3.0408,P<0.05)。辐射后6h和7d大鼠海马COX Ⅰ蛋白表达均较假辐射组减少,给药后3d(辐射后6h)与辐射后6h相比COX Ⅰ蛋白表达升高(t=2.9614,P<0.05),给药后10d(辐射后7d)与辐射后7d相比改变不显著。结论FDP对微波辐射后大鼠海马线粒体呼吸链相关基因COX表达降低有一定的促恢复作用。 |
| 英文摘要: |
| Objective To investigate the effects of fructose-1,6-diphosphate (FDP) on the gene COX expression of mitochondrial respiratory chain in rats hippocampus after microwave exposure.Methods 25 male rats were exposed to microwave that the average power density was 30 mW/cm2. FDP was injected intraperitoneally once per day 3 d before exposure, lasting 3ds or 10ds. Rats were sacrificed at 6h and 7d after exposure. Reverse transcription-polymerase chain reaction (RT-PCR) and image analysis were used to study the expression of COX Ⅰ, Ⅱ and ⅣmRNA. The COX Ⅰ protein was detected by Western blot and image analysis.Results The expression level of COX Ⅰ, Ⅱ and Ⅳ mRNA were decreased at 6h and 7d after exposure while in administration group the expression level increased especially at 3d after administration (tCOX Ⅰ=3.2205, tCOX Ⅱ=3.5762, tCOX Ⅳ=3.0408, P<0.05). The COX Ⅰ protein level decreased after exposure while it increased at 3 d after administration (t =2.9614, P<0.05), but there was no significant change at 10d after administration.Conclusions FDP could recover the decrease of COX expression level of mitochondrial respiratory chain in rats hippocampus after microwave exposure. |
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