中华放射医学与防护杂志

陈雪,刘莉,董继华,等.参芪扶正液对肺腺癌细胞GLC-82X射线照射后TGF-β₁表达调控的研究[J].中华放射医学与防护杂志,2008,28(4):358-360.CHEN Xue,LIU Li,DONG Ji-hua,et al.Modulation role of Shenqi Fuzheng Injection on transforming growth factor beta 1 (TGF-β₁) expression induced by radiation in human lung adenocarcinoma GLC-82 cells[J].Chin J Radiol Med Prot,2008,28(4):358-360

参芪扶正液对肺腺癌细胞GLC-82X射线照射后TGF-β₁表达调控的研究

Modulation role of Shenqi Fuzheng Injection on transforming growth factor beta 1 (TGF-β₁) expression induced by radiation in human lung adenocarcinoma GLC-82 cells

投稿时间：2008-01-08

DOI：

中文关键词: TGF-β₁ 放射损伤参芪扶正液肺癌细胞(GLC-82)

英文关键词:TGF-β₁ Radiation-induced injury Shenqi Fuzheng Injection Human lung adenocarcinoma GLC-82

基金项目:中国临床肿瘤学科学基金资助项目(Y20050040);湖北省科技厅科技公关项目(2005AA401C25)

作者	单位	E-mail
陈雪	430023 武汉, 华中科技大学附属协和医院肿瘤诊疗研究中心
刘莉	430023 武汉, 华中科技大学附属协和医院肿瘤诊疗研究中心	liulixiehe2004@163.com
董继华	430023 武汉, 华中科技大学附属协和医院中心实验室
丁乾	430023 武汉, 华中科技大学附属协和医院肿瘤诊疗研究中心

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中文摘要:

目的阐明参芪扶正液对肺腺癌细胞株GLC-82 X射线照射后TGF-β₁表达的调控,探讨中药防治放射性肺损伤的分子机制。方法选取人低分化肺腺癌细胞株GLC-82,加入终浓度为1.563 mg/ml参芪扶正液后随机分组:1照射剂量关系组:分别予0、20、30和40 Gy剂量的X射线照射,于照射后6 h提取细胞总RNA。 2时间关系组:接受20 Gy的X射线照射,分别在照射后12、24和48 h提取细胞总RNA。2组均采用RT-PCR法检测TGF-β₁mRNA表达。结果剂量关系组:GLC-82细胞接受X射线照射后TGF-β₁mRNA表达量明显增高,在20 Gy照射后达到高峰,为基础未照射水平细胞的5倍。参芪扶正干预组与单纯照射组相比,其TGF-β₁mRNA相对表达量均有不同程度下调,在吸收剂量达20 Gy时差异有统计学意义(P＜0.05)。时间关系组:GLC-82细胞经20 Gy X射线照射后在不同时间点参芪扶正干预组与单纯照射组相比,其TGF-β₁ mRNA相对表达量均有不同程度下调,受照后48 h 差异有统计学意义(P＜0.01)。结论TGF-β₁在放射性肺损伤发生、发展过程中起着重要作用,而参芪扶正液能明显降低该过程中TGF-β₁表达水平,提示参芪扶正液可能通过调控该细胞因子来起到辐射防护之作用。

英文摘要:

Objective To investigate the ability of Shenqi Fuzheng Injection on release of TGF-β₁ mRNA in human lung adenocarcinoma GLC- 82 cells by ionizing radiation in vitro,and find the regulation mechanism to reduce the radiation-induced lung injury by traditional Chinese drug.Methods GLC-82 cells were cultured in media with serum containing 1.563 mg/ml Shenqi Fuzheng Injection,then TGF-β₁ mRNA after exposure to different irradiation doses(0, 20,30,40 Gy) and time intervals(12,24,48 h) were investigated. The expression of TGF-β₁ mRNA detected semi-quantitatively by RT-PCR.Results For the different irradiation doses group, TGF-β₁ expression were increased after irradiation, which was significantly elevated at 20 Gy compared with unirradiated cells. The GLC-82 cells,treated by Shenqi Fuzheng Injection, revealed a minor radiation mediated TGF-β₁ response on mRNA level, which was significantly different at 20 Gy compared with the irradiated group (P<0.05). For the different time intervals group, following irradiation exposure in 20 Gy, the TGF-β₁ mRNA levels in cells, which were treated by Shenqi Fuzheng Injection, were down-regulated and significantly decrease at 48 h (P<0.01).Conclusions The transforming growth factor beta 1 -TGF-β₁ is a key mediator for the pathogenesis of radiation-induced lung injury. Shenqi Fuzheng Injection down-regulates the radiation- induced TGF-β₁ release, and it might reduce the lung toxicity after thoracic irradiation.

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