| 秦颂兵,王利利,涂彧,周菊英,徐晓婷,沈海林.大鼠全脑照射后早期磁共振波谱与相关蛋白表达的对照研究[J].中华放射医学与防护杂志,2008,28(4):319-322 |
| 大鼠全脑照射后早期磁共振波谱与相关蛋白表达的对照研究 |
| Experimental early stage lesions after whole brain irradiation in rats: 1HMRS in comparison with NSE and S-100 protein |
| 投稿时间：2007-12-20 |
| DOI： |
| 中文关键词: 辐射效应 脑损伤 磁共振波谱 NSE S-100蛋白 |
| 英文关键词:Radiation effects Brain injuries 1HMRS NSE S-100 protein |
| 基金项目:国家自然科学基金资助项目(30670638) |
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| 中文摘要: |
| 目的 探讨质子磁共振波谱(1HMRS)对放射性脑损伤的细胞代谢改变及神经元特异性烯醇化酶(NSE)和S-100蛋白表达早期动态演变价值。方法将成熟的SD大鼠40只随机分为空白对照组和单纯照射组,用6 MeV电子线对大鼠进行20 Gy全脑单次垂直照射,单纯照射组大鼠分别于照射后1、7、14和30 d行1HMRS检查,分析氮乙酰门冬氨酸(NAA)、胆碱(Cho)和肌酸(Cr)等信号强度改变,以积分面积进行比较,计算NAA/Cr和Cho/Cr;各组大鼠检查后处死,随机抽3只取脑组织,用免疫组织化学法测定NSE和S-100蛋白的相对含量,并与对照组进行比较。结果在上述时间点,脑组织NAA/Cr、NSE随照射后观察时间的延长而下降,Cho/Cr、S-100随照射后观察时间的延长而升高。与空白对照组相比,大鼠脑组织NAA/Cr和NSE显著下降(F值分别为19.6、35.15,P<0.05),Cho/Cr和S-100显著升高(F值分别为15.32、17.45,P<0.05)。结论脑组织中NAA/Cr、Cho/Cr和NSE、 S-100的变化是辐射诱导的急性脑损伤的敏感指标,1HMRS可在组织细胞代谢水平发现早期的放射性脑损伤。 |
| 英文摘要: |
| Objective To study the value of 1H magnetic resonance spectroscopy (1HMRS) changes and the expression of NSE and S-100 protein in Sprague-Dawley (SD) rat brain tissue irradiated by 6 MeV electron beam.Methods Forty adult SD rats were randomly divided into 2 groups: blank control and irradiation groups. The irradiation group was irradiated the whole brain of wat with a dose of 20 Gy using 6 MeV electron beam. Then the rats were performed with 1HMRS on days 1,7,14 and 30 after radiation to analyze concentration changes of NAA, Cho and Cr, as compared with integra-area to calculate NAA/Cr and Cho/Cr. Immunohistochemical method was used to measure the contents of S-100 and NSE in brain tissue. Results The expression of S-100 and NSE and the ratio of NAA/Cr and Cho/Cr after whole brain irradiation were time-dependent. Compared with those of control group, the indexes above were changed with the different dose levels at different times after radiation [F=19.6(NAA/Cr), 15.32(Cho/Cr),35.15(NSE) and 17.45 (S-100),P<0.05].Conclusions The changes of NAA/Cr,Cho/Cr,NSE and S-100 on the brain can act as the indexes of acute brain injury induced by radiation. 1HMRS can be used to diagnose the injury of the brain in early stage after whole brain irradiation. |
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