| 邹华伟,谭永刚,张贺英.X射线对肺癌细胞株A549的P57kip2和TGF-β1蛋白表达的影响[J].中华放射医学与防护杂志,2008,28(3):249-251.ZOU Hua-wei,TAN Yong-gang,ZHANG He-ying.Effect of X-irradiation on the protein expression of P57kip2 and TGF-β1 in lung cancer cell stain A549[J].Chin J Radiol Med Prot,2008,28(3):249-251 |
| X射线对肺癌细胞株A549的P57kip2和TGF-β1蛋白表达的影响 |
| Effect of X-irradiation on the protein expression of P57kip2 and TGF-β1 in lung cancer cell stain A549 |
| 投稿时间：2007-09-03 |
| DOI： |
| 中文关键词: X射线 肺癌细胞 p57kip2 TGF-β1 |
| 英文关键词:X-rays Lung cancer cell stain p57kip2 TGF-β1 |
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| 摘要点击次数: 3230 |
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| 中文摘要: |
| 目的 观察不同剂量X射线照射后不同时间A549肺癌细胞株中P57kip2和TGF-β1蛋白表达水平,探讨X射线对p57kip2和TGF-β1的影响及临床意义。方法 按常规方法培养肺癌细胞株A549,实验分5组,照射组分别给予不同剂量X射线(2、4、8和12 Gy)照射,于不同时间(6、12、24、36和48 h)提取蛋白,采用Western blot 检测p57kip2和TGF-β1蛋白的表达水平。结果 X射线可导致肺癌细胞P57kip2蛋白的表达增加,照后12 h达峰值,在2～8 Gy之间,p57随着照射剂量的增加而升高,12 Gy时,其含量较4～8 Gy减少。不同剂量和不同时间P57kip2蛋白的表达,差异有统计学意义(P＜0.05),TGF-β1在照后6h达峰值,之后迅速下降,各不同剂量和不同时间水平之间差异有统计学意义(P＜0.05)。结论 X射线照射可以上调肺癌细胞株A549的p57kip2和TGF-β1蛋白表达,两者有一定的相关性,且在一定剂量范围内呈剂量依赖性,可以反映肺癌细胞损伤的程度。 |
| 英文摘要: |
| Objective To analyze the effect of X-irradiation on the proteins expression of p57kip2 and TGF-β1 in lung cancer cell stain A549 and its clinical significance. Methods Lung cancer cell stain A549 was cultivated and cell, protein was extracted at 6,12,24,36 and 48 hours after X-irradiation by different doses(2,4,8 and 12 Gy). The expression of p57kip2 and TGF-β1 proteins were examined by Western blot. Results The expression of p57kip2 in lung cancer cell stain A549 was very low before X-irradiation, and increased significantly after irradiation with different doses and reached the peak level at 12 hours after irradiation(P<0.05). TGF-β1 reached its peak level at 6 hours after irradiation(P＜0.05). Conclusions X-irradiation can up-regulate the expression of p57kip2 and TGF-β1 proteins which increased with certain doses. p57kip2 and TGF-β1 could be used to predict the damage degree of cancer cells by X-ray. |
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