| 张立群,马增春,王宇光,等.雌二醇衍生物对人淋巴母细胞的辐射防护作用及相关机制[J].中华放射医学与防护杂志,2008,28(3):231-235.ZHANG Li-qun,MA Zeng-chun,WANG Yu-guang,et al.Radioprotective effect of E0703 on AHH-1 cells and the related mechanism[J].Chin J Radiol Med Prot,2008,28(3):231-235 |
| 雌二醇衍生物对人淋巴母细胞的辐射防护作用及相关机制 |
| Radioprotective effect of E0703 on AHH-1 cells and the related mechanism |
| 投稿时间：2007-05-28 |
| DOI： |
| 中文关键词: 雌二醇衍生物 电离辐射 AHH-1 细胞周期 辐射防护 |
| 英文关键词:E0703 Ionizing radiation AHH-1 Cell cycle Radioprotection |
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| 中文摘要: |
| 目的 探讨雌二醇衍生物(E0703)对人淋巴母细胞(AHH-1)的辐射防护作用及其可能的作用机制。方法 细胞分为未经任何处理(N)、3.0 Gy、3.0 Gy+E0703(5、20、80和320 nmol/L)6个组。Cell Counting Kit-8试剂盒检测细胞活力,Annexin V -FITC细胞凋亡试剂盒及PI单染检测细胞凋亡及细胞周期,RT-PCR检测mRNA水平变化,Western blot检测蛋白水平变化。结果 AHH-1细胞受到3.0 Gy 60Coγ射线照射以后,细胞活力显著下降,细胞周期明显阻滞,并伴有大量细胞坏死及凋亡。照射前12h给予E0703(5～320 nmol/L)可提高受照细胞的活力、缓解细胞周期阻滞,但对细胞坏死、凋亡影响不显著。细胞受到3.0 Gy照射以后,细胞周期阻滞相关分子Rb、MDM2水平明显升高,加入E0703以后其水平下调,Rb蛋白的磷酸化水平升高。结论 E0703可能通过影响细胞周期和细胞增殖而发挥其辐射防护作用。 |
| 英文摘要: |
| Objective E0703 is derived from estradiol and has anti-radiation effects on irradiated mice, the effects of E0703 on cell viability, cell cycle and the related mechanism in irradiated AHH-1 cells were investigated in this study. Methods Cell viability, cell apoptosis and cell cycle were determined by Cell Counting Kit-8, Annexin V-FITC double staining kit and Flow Cytometry, respectively. Level of mRNA and protein were detected by RT-PCR and Western blot, respectively. Results 3.0 Gy 60Coγ-rays induced significant cell cycle arrest, necrosis, apoptosis and reduction of viability. Pretreatment with E0703 for 12h before irradiation could increase cell viability and regulate cell cycle, but had no obvious effect on cell necrosis and apoptosis. The expression of cell cycle arrest related molecules Rb and MDM2 were increased after 3.0 Gy irradiation, but decreased significantly with pretreatment of E0703. Moreover, the phosphorylation of Rb was also increased. Conclusions The radioprotective function of E0703 might be exerted through influencing on cell cycle and promoting cell proliferation instead of apoptosis or necrosis. |
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