| 张勇,张林生,郭朝华,等.CD34+脐血干细胞转染pIRES2-FL-IL-3后静脉移植对辐射损伤小鼠的影响[J].中华放射医学与防护杂志,2008,28(3):228-231.ZHANG Yong,ZHANG Lin-sheng,GUO Chao-hua,et al.Effect of CD34+ cord blood stem cell transfected by plasmid vector pIRES2-FL-IL-3 on the mice after irradiation[J].Chin J Radiol Med Prot,2008,28(3):228-231 |
| CD34+脐血干细胞转染pIRES2-FL-IL-3后静脉移植对辐射损伤小鼠的影响 |
| Effect of CD34+ cord blood stem cell transfected by plasmid vector pIRES2-FL-IL-3 on the mice after irradiation |
| 投稿时间：2007-06-25 |
| DOI： |
| 中文关键词: 白介素-3 Flt3配体 干细胞 辐射 造血 |
| 英文关键词:Interleukin-3 Flt3 ligand Stem cell Radiation Haematogenesis |
| 基金项目: |
|
| 摘要点击次数: 3831 |
| 全文下载次数: 2486 |
| 中文摘要: |
| 目的 观察CD34+ 脐血干细胞转染质粒载体pIRES2-FL-IL-3后静脉移植对辐射损伤小鼠的影响并探讨其机制。方法 质粒载体pIRES2-FL-IL-3转染脐血CD34+干细胞,将其通过静脉移植到辐射损伤的小鼠体内(5×105个细胞/只,双表达组,12只),观察辐照后2、4和6周动物存活率及外周血象变化。6周时免疫荧光法检测实验小鼠脾脏CD34表达,RT-PCR法和Western blot 法进行IL-3和FL的mRNA和蛋白活性检测,另外3组为单纯CD34+细胞组(CD34组)、转染pIRES2-IL-3CD34+细胞组(IL-3组)和转染pIRES2-FL CD34+细胞组(FL组),各组动物数均为12只。结果 CD34组、IL-3组和FL组动物6周存活率分别为25.0%、50.0%和50.0%,双表达组动物存活率为91.7%,显著高于其余各组。2周时双表达组白细胞数量、红细胞数量及血小板数量显著高于其余3组。双表达组脾脏CD34免疫荧光强度显著高于其余3组,IL-3及FL的mRNA 水平及蛋白活性均显著高于其余3组。结论 真核共表达质粒载体pIRES2-FL-IL-3转染CD34+脐血干细胞后对辐射损伤小鼠具有显著的促脾脏造血作用,其机制与移植后小鼠脾脏组织中移植CD34+脐血干细胞的聚集、增殖及目的 蛋白的高效表达有关。 |
| 英文摘要: |
| Objective To observe the effect of CD34+ cord blood stem cell transfected by plasmid vector pIRES2-FL-IL-3 on the mouse after irradiation and to investigate its mechanisms. Methods In the co-expressed group (12 mice), CD+34 cord blood stem cells were transfected by plasmid vector pIRES2-FL-IL-3. 5×105 cells were injected intravenously in the mouse. The hemogram changes in mice were detected 2, 4 and 6 weeks after radiation. At 6 weeks after irradiation, the expression of the CD34 in spleen was detected by immumofluorescence method. The mRNA level and the activity of IL-3 and FL were detected by RT-PCR and Western blot. Other 3 groups were CD34+ cell group (CD34 group), pIRES2-IL-3 group(IL3 group) and pIRES2-FL group(FL group),and there were 12 mice in each group. Results The survival rate of CD34 group, IL3 group and FL group at the 6th week were 25.0% (3/12), 50.0% (6/12) and 50.0% (6/12), respectively. It was 91.7%(11/12) in the co-expressed group, which was higher than those in the other groups. The expression of the CD34 of spleen in the co-expressed group was higher than those of the other groups. The mRNA level and the activity of IL-3 and FL of spleen in the co-expressed group were higher than those in the other groups too. Conclusions The CD34+ cord blood stem cells transfected by plasmid vector pIRES2-FL-IL-3 have hemogenesis promotion effect on the mice after irradiation, which was related with the aggregation, proliferation of stem cells and the high expression of the interest proteins. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|