| 俞泽阳,范我,吴锦昌,等.重组人p53腺病毒注射液对人淋巴瘤细胞的作用及辐射敏感性的影响[J].中华放射医学与防护杂志,2008,28(3):213-216.YU Ze-yang,FAN Wo,WU Jin-chang,et al.Effect of recombinant adenovirus encoding human p53 tumor suppressor gene (rAd-p53) on the growth and radiotherapeutic sensitivity of human lymphoma celllines[J].Chin J Radiol Med Prot,2008,28(3):213-216 |
| 重组人p53腺病毒注射液对人淋巴瘤细胞的作用及辐射敏感性的影响 |
| Effect of recombinant adenovirus encoding human p53 tumor suppressor gene (rAd-p53) on the growth and radiotherapeutic sensitivity of human lymphoma celllines |
| 投稿时间：2007-12-28 |
| DOI： |
| 中文关键词: 重组人p53腺病毒注射液 p53基因 人淋巴瘤细胞 放射治疗 |
| 英文关键词:rAd-p53 p53 gene Human lymphoma cell lines Radiation therapy |
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| 中文摘要: |
| 目的 探讨重组人p53腺病毒注射液对人淋巴瘤细胞的抑制作用及其辐射增敏性。方法 利用四氮唑盐(MTT)比色法分析重组人p53腺病毒注射液、放射治疗以及两者联合应用对人淋巴瘤细胞Raji 和Daudi的抑制作用;通过蛋白质的Western印迹分析法(Western blotting)分析淋巴瘤细胞内P53蛋白的表达;应用反转录-聚合酶链反应(RT-PCR)检测淋巴瘤细胞内p53基因的mRNA表达。结果 MTT结果显示重组人p53腺病毒注射液对人淋巴瘤细胞具有一定的抑制作用,但抑制作用不显著,Raji 及Daudi 细胞最大剂量的抑制率分别为27.5%±4.1%和28.1%±1.6%,也未见明显的辐射增敏效应。Western blotting和RT-PCR结果表明,外源性P53蛋白和p53基因的mRNA均有所表达,但未见高效表达和明显的辐射增敏性。结论 重组人p53腺病毒注射液对人淋巴瘤细胞的抑制作用及辐射增敏性不显著。 |
| 英文摘要: |
| Objective To explore the inhibitory effect and radiation sensitization of recombinant adenovirus encoding human p53 tumor suppressor gene (rAd-p53) on human lymphoma cell lines. Methods Human lymphoma cell lines Raji and Daudi were treated with rAd-p53, radiation therapy and combined treatment,respectively. The cell growth inhibition was assessed by MTT. The p53 protein expression was detected by Western blotting, and p53 mRNA was detected by RT-PCR. Results The MTT results showed that the inhibitory effect and radiosensitivity enhancement of rAd-p53 on human lymphoma cell lines were not obvious ［Raji:(27.5±4.1)%;Daudi:(28.1±1.6)%］. The results of Western blotting and RT-PCR showed that extrinsic p53 protein and p53 mRNA were expressed to some degree, but not at high-level. In addition, the results didn't demonstrate obvious radiosensitivity enhancement. Conclusions The role of inhibition and radiosensitivity enhancement of rAd-p53 was not significant on human lymphoma cell lines. |
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