| 封巍,祝淑钗,郑晓,等.阻断血管内皮生长因子表达对食管癌细胞放射敏感性的影响[J].中华放射医学与防护杂志,2008,28(2):145-148.FENG Wei,ZHU Shu-chai,WANG Yu-xiang,et al.Effect of antisence VEGF on the radiosensitivity of esophageal cancer cells in vitro[J].Chin J Radiol Med Prot,2008,28(2):145-148 |
| 阻断血管内皮生长因子表达对食管癌细胞放射敏感性的影响 |
| Effect of antisence VEGF on the radiosensitivity of esophageal cancer cells in vitro |
| 投稿时间：2007-01-31 |
| DOI： |
| 中文关键词: 血管内皮生长因子 食管癌 放射敏感性 |
| 英文关键词:VEGF Esophageal cancer Radiosensitivity |
| 基金项目:河北省自然科学基金资助项目(C2005000802),浙江省省医药卫生科学研究基金项目(C2006B011) |
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| 中文摘要: |
| 目的 观察转染反义VEGF cDNA质粒和反义寡核苷酸后,TE-1食管癌细胞在体外的生长状况、VEGF表达水平及其放射敏感性的变化。方法 分别用反义VEGF cDNA质粒及空载体质粒和反义VEGF寡核苷酸经LipofectamineTM2000介导转染TE-1细胞,然后经γ射线照射。采用RT-PCR、Western blotting、流式细胞术、MTT法和克隆形成实验分别检测4组细胞照射前后VEGF基因的表达、凋亡率和细胞周期变化、细胞增殖情况以及转染细胞放射敏感性的变化。结果 将反义VEGF cDNA和反义寡核苷酸成功转入食管癌TE-1细胞后,VEGF表达水平明显降低,细胞增殖反应、细胞周期无明显变化,亦未见明显凋亡发生。照射后4组细胞增殖情况未见明显差异;反义组细胞凋亡率略有上升, 放射敏感性增加。结论 转染反义VEGF cDNA质粒和反义寡核苷酸可抑制食管癌TE-1细胞VEGF表达,联合放射线作用,对TE-1细胞的增殖无明显影响,而对其放射敏感性有增强作用。 |
| 英文摘要: |
| Objective To investigate the effect of antisense VEGF on the cell proliferation, VEGF protein expression and radiosensitivity of esophageal cancer cells in vitro. Methods Fragments of antisense cDNA,empty vector plasmid DNA and antisense oligodeoxynucleotide of VEGF were transfected into esophageal cancer (TE-1)cells mediated with lipofectamine, respectively. Cell proliferating rate and apoptotic rate of these groups were edetected by MTT and FCM methods, respectively. After irradiation, the expression of VEGF in transfected cells were detected by using RT-PCR and Western blotting. The radiosensitivity of transfected cells were analyzed with colony forming assay.Results After antisense cDNA plasmid and antisense oligodeoxynucleotide of VEGF were transfected successfully into TE-1 cells, expressions of VEGF protein decreased,however,the changes in cell growth rate and distribution of cell cycle, and the apoptotic rate were not observed in these transfected cells.After irradiation, the radiosensitivity of transfected TE-1 cells were increased,but there was no significant difference in cell growth rate among groups. The apoptotic rates in antisense groups increased slightly compared to TE-1 and TE-1-E groups.Conclusions Expression of VEGF mRNA and VEGF protein were significantly suppressed in TE-1 cells transfected by antisense cDNA and antisense oligodeoxynucleotide of VEGF. After irradiation, the radiosensitivity of the transfected TE-1 cells was increased. |
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