| 祝宝让,蔡建明,唐古生,等.高效反义STAT3对A549细胞辐射敏感性的影响[J].中华放射医学与防护杂志,2008,28(2):124-127.ZHU Bao-rang,CAI Jian-ming,TANG Gu-sheng,et al.Radiosensitivity of A549 cells transfected by effective STAT3 antisense oligodeoxynucleotides[J].Chin J Radiol Med Prot,2008,28(2):124-127 |
| 高效反义STAT3对A549细胞辐射敏感性的影响 |
| Radiosensitivity of A549 cells transfected by effective STAT3 antisense oligodeoxynucleotides |
| 投稿时间：2007-04-14 |
| DOI： |
| 中文关键词: 反义STAT3 A549 辐射敏感性 凋亡 肿瘤治疗 |
| 英文关键词:STAT3 antisense oligodeoxynucleotides A549 Radiosensitivity Apoptosis Tumor therapy |
| 基金项目:国家自然科学基金资助项目(30470415) |
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| 中文摘要: |
| 目的 探讨高效反义STAT3转染肺腺癌A549细胞后,肿瘤细胞辐射敏感性的变化,为提高恶性肿瘤的辐射敏感性提供新的探索思路和途径。方法 用自行设计成功的高效反义STAT3(AS10)转染A549细胞后,以不同剂量γ射线照射,通过CCK-8试剂盒检测细胞增殖变化,Hoechst 33258染色对细胞凋亡作形态学上的观察;用Annexin V/PI复染,流式细胞仪检测细胞早期凋亡率的变化;Western blot检测STAT3蛋白表达及磷酸化变化情况,以及其下游基因表达变化情况。结果高效反义STAT3(AS10)转染后,加以γ射线照射,A549细胞的增殖相比于单独作用组受到明显抑制,细胞早期凋亡水平也增加,STAT3蛋白及其下游Bcl-Xl、Cyclin D1蛋白表达变化明显下降,STAT3蛋白磷酸化水平也降低。结论 反义STAT3(AS10)联合γ射线对A549细胞的增殖抑制和诱导凋亡作用明显增强,提高了A549细胞的辐射敏感性;表明阻断STAT3蛋白表达可能成为一种新的提高肿瘤辐射敏感性的有效手段。 |
| 英文摘要: |
| Objective To investigate the influence of effective STAT3 antisense oligodeoxynucleotides(AS10) on radiosensitivity of A549 cells.Methods Oligodeoxynucleotides were tansfected into A549 cells.Cell viability was assessed using CCK-8 assay. Apoptotic assay was performed using Hoechst 33258 staining and Annexin V/PI with FACS analysis, the expressions of STAT3, P-STAT3, Bcl-Xl and Cyclin D1, were measured by Western blot.Results The expression of STAT3、P-STAT3、Bcl-Xl and Cyclin D1 were downregulated.Cell viability was decreased significantly in those groups transfected with AS10 followed by different dose of γirradiation. Fraction of early apoptosis was increased when combined AS10 transfection with irradiation compared with those cells treated with irradiation alone or NS transfection with subsequent exposure to irradiation.Conclusion These results indicate that effective STAT3 antisense oligodeoxynucleotides(AS10) can enhance the therapeutic effect of γ-irradiation to A549 cells, which support the role of STAT3 as a potential molecular target for tumor therapies, and also a potential molecular target for enhancement of radiosensitivity of radioresistant tumors. |
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