| 刁瑞英,宋良文,王少霞,等.放射性肺损伤早期肺组织重建机制的探讨[J].中华放射医学与防护杂志,2008,28(2):117-119,131.DIAO Rui-ying,SONG Liang-wen,WANG Shao-xia,et al.Exploration on remodeling of lung tissue in early radiation pulmonary injury[J].Chin J Radiol Med Prot,2008,28(2):117-119,131 |
| 放射性肺损伤早期肺组织重建机制的探讨 |
| Exploration on remodeling of lung tissue in early radiation pulmonary injury |
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| DOI： |
| 中文关键词: 照射 成纤维细胞 巨噬细胞 Ⅳ型胶原 肺损伤重建 |
| 英文关键词:Irradiation Fibroblast Macrophage Collagen type Ⅳ Pulmonary remodeling |
| 基金项目:国家自然科学基金资助项目(30570545) |
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| 中文摘要: |
| 目的 探讨Ⅳ型胶原和MMP-9在放射性肺损伤早期肺组织重建中的作用。方法 用噻唑蓝(MTT)检测1～10 Gy 60Co γ 射线照射对人肺成纤维细胞(Fb)增殖的影响;用酶联免疫吸附法测定经5和7 Gy照射后Fb中Ⅳ型胶原和MMP-9的变化;从经25 Gy照射后大鼠肺泡灌洗液中分离巨噬细胞,制备条件培养液(CMAM)并刺激肺Fb,用MTT检测对细胞增殖的影响;同时用ELISA测定对Fb合成Ⅳ型胶原和MMP-9的影响;于照射后不同时间取大鼠肺组织进行Ⅳ型胶原和MMP-9免疫组化染色。结果 1～7 Gy照射能促进肺Fb增生;5和7 Gy照射能促进Fb合成MMP-9,但不能促进Ⅳ型胶原合成;CMAM不但能促进Fb增生和MMP-9合成,也能合成和释放Ⅳ型胶原;受照后1周大鼠肺组织出现Ⅳ型胶原沉积。结论 照射能直接刺激肺Fb增生,但不能产生Ⅳ型胶原;Ⅳ型胶原的合成与照射后肺内巨噬细胞和Fb的相互作用有关,并可导致肺损伤重建的发生。 |
| 英文摘要: |
| Objective To investigate the roles of collagen type Ⅳ and Matrix metalloproteinase-9 (MMP-9) on remodeling of lung tissue in early radiation pulmonary injury.Methods The proliferation of human lung fibroblast (Fb) was determined by MTT following irradiation with 60Co γ ray of 1-10 Gy; the alteration of collagen type Ⅳ and MMP-9 was measured by ELISA following Fb was irradiated with 5 and 7 Gy;Macrophage was isolated from alveolar lavage solution of rat irradiated by 25 Gy of γ ray, and condition medium of alveolar macrophage (CMAM) was prepared for stimulation of pulmonary Fb, and the cellular proliferation was determined by MTT, the synthesis of collagen type Ⅳ and MMP-9 was measured by ELISA. Immunohistochemical staining of collagen type Ⅳ and MMP-9 was performed with rat lung tissue at different times after irradiation.Results Irradiation with 1-7 Gy on pulmonary Fb could promote cellular proliferation and MMP-9 synthesis, but could not promote the collagen type Ⅳ synthesis. However, the CMAM could not only promote Fb proliferation and MMP-9 synthesis, but also promote collagen type Ⅳ synthesis and release. The deposition of collagen type Ⅳ in lung tissue could be found one week after irradiation.Conclusions Radiation can promote pulmonary Fb proliferation but can't make it produce collagen type Ⅳ. The synthesis of collagen type Ⅳ is related to interaction between pulmonary macrophage and Fb after irradiation, and it is directly involved in pulmonary remodeling after radiation pulmonary injury. |
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