中华放射医学与防护杂志

刘青杰,陆雪,封江彬,等.BACFISH与PAINT法检测辐射诱发染色体易位的比较[J].中华放射医学与防护杂志,2008,28(2):112-116.LIU Qing-jie,LU Xue,FENG Jiang-bin,et al.Comparison of BAC FISH with specific telomeres and centromere probes and chromosome painting on detection of chromosome translocation induced by irradiation[J].Chin J Radiol Med Prot,2008,28(2):112-116

BACFISH与PAINT法检测辐射诱发染色体易位的比较

Comparison of BAC FISH with specific telomeres and centromere probes and chromosome painting on detection of chromosome translocation induced by irradiation

投稿时间：2007-04-05

DOI：

中文关键词: ⁶⁰Co γ射线染色体易位 BAC FISH 染色体涂染剂量-效应曲线

英文关键词:⁶⁰Co γ rays Chromosome translocation BAC FISH PAINT Dose-response curve

基金项目:国家自然科学基金资助项目(30570551);北京市自然科学基金资助项目(7053073);公共卫生专项(54006-0803)

作者	单位	E-mail
刘青杰	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所	qjliu@nirp.cn
陆雪	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
封江彬	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
王晓维	国家人类基因组北方研究中心
陈德清	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所

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中文摘要:

目的比较自行建立的BAC FISH方法和常规染色体涂染(PAINT)方法分析辐射诱发染色体易位有效性的不同。方法对正常人外周血照射不同剂量(0～5.0 Gy)的⁶⁰Co γ射线,用1、2和4号染色体特异性端粒和着丝粒探针BAC FISH及PAINT分析染色体易位,将观察到的染色体易位率换算为全基因组易位率,并建立两种方法分析辐射诱发的染色体易位率剂量-效应曲线。结果用两种方法分析0～5.0 Gy ⁶⁰Co γ射线诱发的全基因组易位率均随着吸收剂量的增加而增高;在相同的剂量点,BAC FISH染色体易位检出率高于PAINT方法。两种方法分析吸收剂量和全基因组易位率之间的剂量-效应曲线均为二次方程模式,分别为Y= 0.043D²+0.0008D+0.0048(BAC FISH)和Y=0.043D²+0.006D+0.0027 (PAINT)。结论自行建立的BAC FISH方法分析辐射诱发染色体易位检出率高于常规染色体涂染方法,两种方法建立的剂量-效应曲线方程的β系数相同。

英文摘要:

Objective To compare the efficiency of BAC FISH established in our lab and conventional chromosome painting (PAINT) on detection of radiation-induced chromosome translocation.Methods Healthy human peripheral blood samples were irradiated with 0～5.0 Gy ⁶⁰Co γ-rays. Then chromosome translocations in these samples were detected with BAC FISH and PAINT using chromosomes 1, 2 and 4. The genome translocation rates were calculated with observed chromosome translocation rates, and the dose-response curve of two methods were established.Results The genome translocation rates induced by 0～5.0 Gy ⁶⁰Co γ-rays detected by BAC FISH and PAINT were increased with absorbed doses. The observed translocation rates with BAC FISH were higher than that with PAINT at each dose level. The dose-response curve were Y= 0.043D² +0.0008D +0.0048 for BAC FISH and Y=0.043D²+0.006D+0.0027 for PAINT.Conclusions The translocation rate detected by BAC FISH was higher than that by PAINT, and the parameters β in dose-response curve equation were same by two methods.

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