中华放射医学与防护杂志

夏曙,于世英,赵茵,等.PI3KAktCOX-2途径在人宫颈癌HeLa细胞放射抗拒中的作用[J].中华放射医学与防护杂志,2008,28(2):108-111.XIA Shu,ZHAO Yin,et al.Role of PI3K Akt COX-2 pathway in the radiation resistance of human cervical cancer HeLa cells[J].Chin J Radiol Med Prot,2008,28(2):108-111

PI3KAktCOX-2途径在人宫颈癌HeLa细胞放射抗拒中的作用

Role of PI3K Akt COX-2 pathway in the radiation resistance of human cervical cancer HeLa cells

投稿时间：2007-06-04

DOI：

中文关键词: PI3K Akt Hela细胞放射敏感性 X射线塞来昔布

英文关键词:PI3K Akt HeLa cell Radiosensitivity X_rays Celecoxib

基金项目:国家自然科学基金资助项目(30672426)

作者	单位
夏曙	430030 武汉, 华中科技大学同济医学院附属同济医院肿瘤中心
于世英	430030 武汉, 华中科技大学同济医学院附属同济医院肿瘤中心
赵茵	430030 武汉, 华中科技大学同济医学院附属同济医院肿瘤中心
郑微	430030 武汉, 华中科技大学同济医学院附属同济医院肿瘤中心

摘要点击次数: 3173

全文下载次数: 2658

中文摘要:

目的探讨PI3K/Akt/COX-2途径在人宫颈癌HeLa细胞放射抗拒中作用的分子机制。方法 COX-2抑制剂塞来昔布单独及联合PI3K抑制剂LY294002作用HeLa细胞24 h,X射线不同剂量照射,利用克隆形成法计算细胞存活率,单击多靶模型拟合细胞存活曲线,计算D_q、D₀、SF₂值和放射增敏比(SER);应用Western blot方法检测Akt、磷酸化Akt、COX-2、Bad和磷酸化Bad蛋白的表达;应用RT-PCR检测COX-2和Bad mRNA的表达。结果塞来昔布单独及联合LY294002作用HeLa细胞组D_q、D₀、SF₂明显低于单纯照射组;X射线照射后,能够活化HeLa细胞PI3K/Akt/COX-2途径,塞来昔布能够多靶点抑制PI3K/Akt/COX-2途径的活化。结论 PI3K/Akt/COX-2途径的活化是HeLa细胞放射抗拒的重要原因,PI3K/Akt与COX-2之间存在正反馈的调节,抑制PI3K/Akt/COX-2途径的活化能够提高HeLa细胞放射敏感性。

英文摘要:

Objective To explore the role of PI3K/Akt/COX-2 pathway in the radiation resistance of human cervical cancer HeLa cells.Methods The HeLa cells were cultured in vitro. Using Celecoxib in Hela cells or associated with LY294002 for 24h, the cells were radiated by different doses of X-ray. The cell survival ratio was detected by clone formation. To calculate D_q, D₀, SF₂, SER, the cell survival curve was draw by one-hit multitarget model. The expression of pAkt, Akt, COX-2, Bad and pBad were detected by Western blot. The mRNA expression of COX-2 and Bad was detected by RT-PCR.Results The D_q、D₀、SF₂ of Celecoxib or associated with LY294002 group was lower than those in radiation group. The pathway PI3K/Akt/COX-2 was activated by irradiation. Celecoxib inhibited the activation of PI3K/Akt/COX-2 for multitarget.Conclusions The activation of PI3K/Akt/COX-2 signal transduction resulted in resistance of radiosensitization in HeLa cell. It was indicated that inhibiting PI3K/Akt/COX-2 pathway could improve the radiosensitivity of human cervical cancer HeLa cells.

HTML 查看全文查看/发表评论下载PDF阅读器

关闭