中华放射医学与防护杂志

徐胜利,沈丽,陈彪.⁶⁰Coγ射线诱导的新生大鼠大脑皮质神经细胞凋亡[J].中华放射医学与防护杂志,2008,28(2):99-103.XU Sheng-li,SHEN Li,CHEN Biao.Apoptosis of neural cells in the neonatal rat cerebral cortex induced by ⁶⁰Co Gamma Ray[J].Chin J Radiol Med Prot,2008,28(2):99-103

⁶⁰Coγ射线诱导的新生大鼠大脑皮质神经细胞凋亡

Apoptosis of neural cells in the neonatal rat cerebral cortex induced by ⁶⁰Co Gamma Ray

投稿时间：2007-05-29

DOI：

中文关键词: 电离辐射大脑皮质凋亡 p53 诱导型一氧化氮合酶

英文关键词:Ionizing radiation Cerebral cortex Apoptosis p53 iNOS

基金项目:国家自然科学基金(30371574,30430280,30671950)

作者	单位	E-mail
徐胜利	100053 北京, 首都医科大学宣武医院, 北京市老年病研究所神经生物研究室, 教育部神经退行性疾病重点实验室
沈丽	北京大学医学部细胞生物学系	shenli@bjmu.edu.cn
陈彪	100053 北京, 首都医科大学宣武医院, 北京市老年病研究所神经生物研究室, 教育部神经退行性疾病重点实验室

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中文摘要:

目的采用电离辐射诱导的新生大鼠大脑皮质神经细胞损伤模型,探讨电离辐射诱导发育脑神经细胞死亡的特征和机制。方法新生大鼠接受单次2.0 Gyγ射线照射后,采用DNA凝胶电泳、TUNEL和HE染色鉴定大脑皮质神经细胞死亡的类型和时程变化特点;应用免疫组织化学方法研究p53和iNOS在细胞死亡过程中的可能作用。结果 DNA凝胶电泳、TUNEL和HE染色表明,2.0 Gyγ射线照射可诱导新生大鼠大脑皮质神经细胞凋亡。大脑皮质各区域凋亡指数于照射后4 h开始增高,至12 h达到峰值;照射后相同时间点各皮质区域凋亡指数比较,新皮质最高,海马次之,旧皮质最低;P53和iNOS免疫阳性细胞计数定量结果显示,照射后相同时间点各皮质区域P53和iNOS免疫阳性细胞数差异无统计学意义。结论 2.0 Gyγ射线照射诱导了新生大鼠大脑皮质神经细胞凋亡;不同皮质区域的神经细胞对电离照射引起的损伤反应是相似的,但不同皮质区域的神经细胞凋亡存在差异。

英文摘要:

Objective To identify the cell death type and investigate the potential mechanism of ionizing radiation-induced neural cell death in the neonatal rat cerebral cortex.Methods The neonatal Wistar rats were given a single dose of 2.0 Gyγ_irradiation. The cell death type and characterization in cerebral cortex were identified using DNA electrophoresis, TUNEL and HE staining. The P53- and iNOS-positive cells were analyzed quantitatively using immunohistochemistry.Results The DNA and morphological characterization of death cells indicated that 2.0 Gyγ-irradiation induced apoptosis of the neural cells in neonatal rat cerebral cortex. The apoptosis indices in different cortex regions were significantly increased 4 h after irradiation, and reached the peak value at 12 h post-irradiation. The apoptosis index of neocortex was much higher than that of hippocampus (archicortex) and paleocortex, while paleocortex had lower apoptosis index than hippocampus. The quantitative immunohistochemistry suggested that the numbers of P53 and iNOS-positive cells were not different between these three cortex regions at the same time-point after irradiation.Conclusion 2.0 Gyγ-rays induced apoptosis of the neural cells in neonatal rat cerebral cortex. The response of cells to the damage effects of ionizing radiation was similar in different cortex regions; however, the apoptosis indices were different significantly. These findings imply that the developing phase or type of neural cells may play a pivotal role in the apoptosis process induced by ionizing radiation.

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