| 方廖琼,何成明,傅晓岚,牛荣,魏泓.羊胎盘免疫调节因子对 60Co γ射线致免疫损伤小鼠T细胞免疫功能的促进作用[J].中华放射医学与防护杂志,2008,28(1):46-49 |
| 羊胎盘免疫调节因子对 60Co γ射线致免疫损伤小鼠T细胞免疫功能的促进作用 |
| Immunoenhancement effect of goat placenta immunoregulating factor on T cell function in immunodeficiency BALB/c mice induced by 60Co γ-rays |
| 投稿时间：2006-12-05 |
| DOI： |
| 中文关键词: 羊胎盘免疫调节因子 辐射损伤 T细胞功能 免疫促进 |
| 英文关键词:Goat placenta immunoregulating factor Radiation injury T cell function Immunoenhancement |
| 基金项目: |
|
| 摘要点击次数: 3530 |
| 全文下载次数: 1891 |
| 中文摘要: |
| 目的 研究羊胎盘免疫调节因子对60Co γ射线辐射所致免疫损伤小鼠T细胞功能的影响。方法 5 Gy 60Co γ射线一次性全身照射建立BALB/c小鼠免疫损伤动物模型,MTT法测定ConA 诱导的T淋巴细胞的增殖反应;直接免疫荧光抗体标记,FACS分析测定脾淋巴细胞CD3、CD4、CD8阳性细胞百分率、胸腺淋巴细胞CD4 CD8亚群细胞数目;ELISA法检测血清IL-2、IFN-γ水平。结果 羊胎盘免疫调节因子显著促进ConA 诱导的T淋巴细胞增殖,提高60Co γ射线所致免疫损伤小鼠CD3+、CD4+和CD8+阳性细胞百分率及胸腺细胞CD4+ CD8-和CD4- CD8+单阳性亚群数目,减少胸腺细胞CD4+ CD8+双阳性亚群数目,促进小鼠血清IL-2、IFN-γ的生成水平。结论 羊胎盘免疫调节因子对60Co γ射线辐射所致免疫损伤小鼠T细胞免疫功能具有促进作用。 |
| 英文摘要: |
| Objective To observe the immunoenhancement effect of goat placenta immunoregulating factor (GPIF) on T cell function in mice with immunodeficiency induced by 60Co γ-rays.Methods Animal models for immunodeficiency were established with BALB/c mice whole-body irradiated by 5 Gy 60Co γ-rays. MTT method was used to evaluate the T lymphopoiesis induced by ConA. FACS were applied to analyze the expression of CD3,CD4 and CD8 in splenic lymphocytes and the rate of CD4 CD8 in thymus lymphocyte. The content of IL-2 and IFN-γin serum were measured by ELISA.Results GPIF could promote T lymphopoiesis of spleen induced by ConA and increase the levels of IL-2 and IFN-γ in serum significantly. The cell percentages of CD3+, CD4+and CD8+ lymphocytes in spleen were significantly increased, and the number of CD4+ CD8- and CD4- CD8+ groups in thymus were increased while the number of CD4+ CD8+ was distinctly decreased with GPIF applied for mice.Conclusions GPIF could facilitate T cell function and promote immunodeficiency mice recovery from radiation injury. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |