中华放射医学与防护杂志

方廖琼,何成明,傅晓岚,等.羊胎盘免疫调节因子对 ⁶⁰Co γ射线致免疫损伤小鼠T细胞免疫功能的促进作用[J].中华放射医学与防护杂志,2008,28(1):46-49.FANG Liao-qiong,HE Cheng-ming,FU Xiao-lan,et al.Immunoenhancement effect of goat placenta immunoregulating factor on T cell function in immunodeficiency BALB/c mice induced by ⁶⁰Co γ-rays[J].Chin J Radiol Med Prot,2008,28(1):46-49

羊胎盘免疫调节因子对 ⁶⁰Co γ射线致免疫损伤小鼠T细胞免疫功能的促进作用

Immunoenhancement effect of goat placenta immunoregulating factor on T cell function in immunodeficiency BALB/c mice induced by ⁶⁰Co γ-rays

投稿时间：2006-12-05

DOI：

中文关键词: 羊胎盘免疫调节因子辐射损伤 T细胞功能免疫促进

英文关键词:Goat placenta immunoregulating factor Radiation injury T cell function Immunoenhancement

基金项目:

作者	单位
方廖琼	400016 重庆医科大学生物医学工程系医学超声研究所
何成明	西南大学生命科学学院
傅晓岚	第三军医大学基础部
牛荣	第三军医大学基础部
魏泓	第三军医大学基础部

摘要点击次数: 4156

全文下载次数: 2113

中文摘要:

目的研究羊胎盘免疫调节因子对⁶⁰Co γ射线辐射所致免疫损伤小鼠T细胞功能的影响。方法 5 Gy ⁶⁰Co γ射线一次性全身照射建立BALB/c小鼠免疫损伤动物模型,MTT法测定ConA 诱导的T淋巴细胞的增殖反应;直接免疫荧光抗体标记,FACS分析测定脾淋巴细胞CD₃、CD₄、CD₈阳性细胞百分率、胸腺淋巴细胞CD₄ CD₈亚群细胞数目;ELISA法检测血清IL-2、IFN-γ水平。结果羊胎盘免疫调节因子显著促进ConA 诱导的T淋巴细胞增殖,提高⁶⁰Co γ射线所致免疫损伤小鼠CD₃⁺、CD₄⁺和CD₈⁺阳性细胞百分率及胸腺细胞CD₄⁺ CD₈^-和CD₄^- CD₈⁺单阳性亚群数目,减少胸腺细胞CD₄⁺ CD₈⁺双阳性亚群数目,促进小鼠血清IL-2、IFN-γ的生成水平。结论羊胎盘免疫调节因子对⁶⁰Co γ射线辐射所致免疫损伤小鼠T细胞免疫功能具有促进作用。

英文摘要:

Objective To observe the immunoenhancement effect of goat placenta immunoregulating factor (GPIF) on T cell function in mice with immunodeficiency induced by ⁶⁰Co γ-rays.Methods Animal models for immunodeficiency were established with BALB/c mice whole-body irradiated by 5 Gy ⁶⁰Co γ-rays. MTT method was used to evaluate the T lymphopoiesis induced by ConA. FACS were applied to analyze the expression of CD₃,CD₄ and CD₈ in splenic lymphocytes and the rate of CD₄ CD₈ in thymus lymphocyte. The content of IL-2 and IFN-γin serum were measured by ELISA.Results GPIF could promote T lymphopoiesis of spleen induced by ConA and increase the levels of IL-2 and IFN-γ in serum significantly. The cell percentages of CD₃⁺, CD₄⁺and CD₈⁺ lymphocytes in spleen were significantly increased, and the number of CD₄⁺ CD₈^- and CD₄^- CD₈⁺ groups in thymus were increased while the number of CD₄⁺ CD₈⁺ was distinctly decreased with GPIF applied for mice.Conclusions GPIF could facilitate T cell function and promote immunodeficiency mice recovery from radiation injury.

HTML 查看全文查看/发表评论下载PDF阅读器

关闭