| 乔俏,张硕,陈延治,李光.蛋白激酶C对人胰腺癌细胞放射敏感性的影响及其机制[J].中华放射医学与防护杂志,2008,28(1):41-43 |
| 蛋白激酶C对人胰腺癌细胞放射敏感性的影响及其机制 |
| Role and mechanism of PKC on radiosensitization in pancreatic carcinoma cell line Panc-1 |
| 投稿时间：2007-03-16 |
| DOI： |
| 中文关键词: 胰腺肿瘤 放射敏感性 蛋白激酶C 凋亡 |
| 英文关键词:Pancreatic neoplasm Radiosensitization Protein kinase C Apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的 研究蛋白激酶C对人胰腺癌细胞Panc-1体外放射敏感性的影响并探讨其机制。方法 应用蛋白激酶C激活剂PMA和抑制剂CH分别观察其对Panc-1细胞存活分数的影响。采用多靶单击数学模型拟合细胞剂量存活曲线,明确CH对人胰腺癌细胞Panc-1的放射增敏效应。采用Annexin Ⅴ/PI法流式细胞仪检测CH对细胞凋亡的影响。采用免疫细胞化学方法检测Bcl-2、Bax蛋白表达的变化。结果 单纯照射组、PMA处理组和CH处理组细胞SF2值分别为0.78±0.02、0.92±0.11和0.19±0.20。浓度为0.5、2和8μmol/L 的CH作用4h后,放射增敏比分别为1.05、1.24和1.77,呈药物浓度依赖性。流式细胞仪检测显示PMA可使照射后肿瘤细胞凋亡指数降低,而CH则使凋亡指数增加。CH作用后,肿瘤细胞Bax蛋白表达水平增加,Bcl-2蛋白表达水平无明显变化,但Bax/Bcl-2值增大。结论 蛋白激酶C通过改变肿瘤细胞的凋亡水平实现对人胰腺癌细胞Panc-1放射敏感性的调控。 |
| 英文摘要: |
| Objective To explore the effect of PKC on radiosensitization in pancreatic carcinoma cell line Panc-1, and its mediating mechanism. Methods Panc-1 cells were treated with the specific activator of PKC (phorbol 12-myristate 13-acetate, PMA) and the specific inhibitor of PKC (chelerythrine, CH) to observe the SF2 changes. Cell survival was determined by clonogenic assay. The apoptosis rates of the cells were analyzed by flow cytometry with Annexin Ⅴ/PI staining. The expression of apoptosis related protein Bcl-2 and Bax after the treatment of CH and/or irradiation was determined by immunocytochemistry. Results The SF2 values of radiation group, PMA group and CH group were 0.78±0.02, 0.92±0.11 and 0.19±0.20, respectively. CH can significantly increase the sensitivity of Panc-1 to irradiation. SERs of Panc-1 cells were 1.05, 1.24 and 1.77 after the treatment of 0.5, 2 and 8 μmol/L of CH, respectively. The result of flow cytometry analysis showed that PMA decreased the apoptosis index with irradiation, while CH significantly increased the apoptosis index. Expression of Bax protein was increased significantly (P<0.05) while that of Bcl-2 was not influenced; however, the ratio of Bax/Bcl-2 was increased. Conclusions PKC regulates the radiosensitivity of Panc-1 by mediating the apoptosis of tumor cells. |
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