| 刁瑞英,宋良文,王少霞,尹纪业.IFN-γ拮抗60Coγ射线刺激肺成纤维细胞增殖的机制研究[J].中华放射医学与防护杂志,2008,28(1):13-16 |
| IFN-γ拮抗60Coγ射线刺激肺成纤维细胞增殖的机制研究 |
| Mechanism of IFN-γ antagonizing pulmonary fibroblast proliferation stimulated by 60Co γ-rays |
| 投稿时间：2006-10-30 |
| DOI： |
| 中文关键词: 人肺成纤维细胞 照射 增殖 TGF-β1 IFN-γ |
| 英文关键词:Human lung fibroblasts Irradiation Proliferation TGF-β1 Interferon-γ |
| 基金项目:国家自然科学基金资助项目(30570545) |
|
| 摘要点击次数: 2854 |
| 全文下载次数: 1654 |
| 中文摘要: |
| 目的 探讨IFN-γ 拮抗60Co γ 射线刺激人肺成纤维细胞(HLF)增殖的机制。方法 应用MTT比色法,观察TGF-β1、受照后大鼠血清促HLF增殖的时效和量效关系及IFN-γ对增殖的影响;用ELISA检测受照后大鼠血清、肺组织TGF-β1水平及IFN-γ 对照后大鼠血清促HLF合成TGF-β1的影响。结果 TGF-β1有促HLF增殖的作用;受照后大鼠血清能促HLF增殖并合成TGF-β1;IFN-γ 显著拮抗血清的上述作用;受照后1～4周大鼠血清和肺组织TGF-β1水平逐渐增加。结论 TGF-β1参与γ 射线刺激HLF增殖的作用,而IFN-γ在一定程度上抑制HLF合成TGF-β1,并拮抗其促HLF增殖的作用。 |
| 英文摘要: |
| Objective To explore the mechanism of IFN-γ antagonizing human pulmonary fibroblasts (HLF) proliferation stimulated by 60Co γ-rays.Methods The time- and dose-effect of HLF proliferation promoted by TGF-β1 and rat serum after radiation as well as effect of IFN-γ on proliferation were observed by MTT colorimetric methods. Content of TGF-β1 in rat serum and pulmonary tissues after irradiation as well as the effect of IFN-γ on rat serum after radiation promoting HLF to synthesize TGF-β1 were determined by ELISA.Results TGF-β1 played a role in promoting HLF proliferation. The sera of irradiated rats could promote HLF proliferation and TGF-β1 synthesis, but the effect was inhibited by IFN-γ significantly (P<0.05). The level of TGF-β1 in rat serum and lung tissues gradually increased from 1 to 4 weeks after irradiation.Conclusions TGF-β1 is involved in the process of γ-rays stimulating HLF proliferation, while IFN-γ can inhibit HLF from synthesizing TGF-β1 and antagonize its role of promoting HLF proliferation. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |