| 金问森,孔肇路,沈芝芬,童顺高,季华均,金一尊.γ射线照射后乏氧诱导因子1α对肝癌细胞存活的影响[J].中华放射医学与防护杂志,2008,28(1):1-4 |
| γ射线照射后乏氧诱导因子1α对肝癌细胞存活的影响 |
| Study on radiation regulation of hypoxia inducible factor-1α expression and its correlation with hepatoma radiosensitivity |
| 投稿时间：2007-07-09 |
| DOI： |
| 中文关键词: 乏氧诱导因子1α 肝癌细胞 放射耐受性 |
| 英文关键词:Hypoxia inducible factor-1α Hepatoma cells Radiation tolerance |
| 基金项目:国家高技术研究发展计划“863”项目(2002AA2Z3104);国家自然科学基金资助项目(30500143,30400089) |
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| 中文摘要: |
| 目的 研究辐射对肝癌细胞内乏氧诱导因子1α(HIF-1α)的调节作用以及HIF-1α的变化对肝癌细胞存活的影响。方法 采用CoCl2化学模拟乏氧预处理人肝癌HepG2细胞,经不同剂量的γ射线照射后,通过细胞集落形成实验,比较有氧及化学模拟乏氧条件下细胞存活水平的差异。同时采用RT-PCR和Western blot方法检测照射后细胞内HIF-1α在转录和翻译水平上的变化。结果 在化学模拟乏氧条件下,细胞存活分数(SF)显著增加,与有氧对照组比较差异有统计学意义,且两种不同作用条件下的SF比值(SFCO/SFO2)随照射剂量逐渐增加,同时,经照射后细胞内的HIF-1α表达升高,并呈剂量依赖模式,HIF-1α的表达与照射后的细胞存活水平具有较强的关联性。结论 辐射可诱导肝癌细胞内HIF-1α的表达升高,HIF-1α的表达变化可能影响细胞的存活水平。 |
| 英文摘要: |
| Objective To study the regulation of hypoxia inducible factor-1α(HIF-1α) expression in hepatoma cells after irradiation and the expression of HIF-1α effect on the radiosensitivity of heptoma cells.Methods HepG2 cells were pretreated by Cobalt chloride (CoCl2), a chemical hypoxia agent, to induce and stabilize the expression of HIF-1α, and then exposed to different γ-irradiation doses. Clonogenic assay was used to evaluate HepG2 cell survival fraction (SF) after irradiation under normoxia and chemical hypoxia. Reverse transcriptase polymerase chain reaction (RT-PCR) and immunoblot assay (Western blot) were utilized to detect the changes of intracellular HIF-1α on the level of transcripation and translation.Results Cell survival level was elevated by chemical hypoxia and there was a statistical difference between chemical hypoxic group and normoxic group. The ratios of SF(SFCO/SFO2)on two different conditions were increased with irradiation doses. Meanwhile, the irradiation induced up-regulation of HIF-1α in dose-dependent manner. The expression of HIF-1α was correlated with HepG2 cell survival level to some extent.Conclusions Irradiation could up-regulate the level of HIF-1α expression in HepG2 cells under chemical hypoxic condition. The cells survival level might be influenced by the changes in HIF-1α expression. |
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