| 赵黎,彭瑞云,高亚兵,等.微波辐射对大鼠海马线粒体形态及能量代谢的影响[J].中华放射医学与防护杂志,2007,27(6):602-604.ZHAO Li,PENG Rui-yun,GAO Ya-bing,et al.Mitochondria morphologic changes and metabolic effects of rat hippocampus after microwave irradiation[J].Chin J Radiol Med Prot,2007,27(6):602-604 |
| 微波辐射对大鼠海马线粒体形态及能量代谢的影响 |
| Mitochondria morphologic changes and metabolic effects of rat hippocampus after microwave irradiation |
| 投稿时间：2006-11-10 |
| DOI： |
| 中文关键词: 微波辐射 大鼠 海马 线粒体 能量代谢 |
| 英文关键词:Microwave radiation Rat Hippocampus Mitochondria Energy metabolism |
| 基金项目:军队医药卫生“十一五”专项基金(06Z065) |
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| 中文摘要: |
| 目的 探讨微波辐射对大鼠海马线粒体形态及能量代谢的影响。方法 采用30 mW/cm2微波辐射30只雄性Wistar大鼠,于辐射后6 h、1 d、3 d和7 d活杀取海马,采用电镜观察线粒体超微结构改变,酶活性染色显示线粒体ATP酶活性改变,分光光度计测定线粒体琥珀酸脱氢酶(succino denhydrogenase,SDH)、单胺氧化酶(monoamine oxidase,MAO)活性,高效液相色谱测量线粒体腺苷酸含量的变化。结果 30 mW/cm2微波辐射后6 h,海马线粒体大小、形状不规则,辐射后1和3 d线粒体损伤加重,出现肿胀、空化、嵴紊乱、短小、数量减少等改变。SDH活性和ATP含量在辐射后6 h降低,3 d降至最低(P<0.01),7 d呈恢复趋势。ATP酶和MAO活性变化一致,于辐射后1和3 d活性升高最为显著(P<0.01)。结论 微波辐射可损伤大鼠海马线粒体结构与功能,使线粒体能量代谢酶异常。 |
| 英文摘要: |
| Objective To investigate the effect of microwave on mitochondria morphologic and metabolism of rat hippocampus. Methods 30 male rats were exposed to microwave with the average power density of 30 mW/cm2. Rats were sacrificed at 6 h, 1 d, 3 d and 7 d after irradiation. Electron microscope, enzymatic activity staining and spectrophotometer were used to study ultrastructure change of hippocampus mitochondria and activity of ATPase, SDH and MAO. Mitochondrial ATP, ADP and AMP contents were measured by high performance liquid chromatography (HPLC).Results At 6 h after microwave radiation, the sizes and shapes of hippocampus mitochondria were abnormal and the injury of mitochondria was aggravated at 1 and 3 d after radiation. The mitochondria presented swell, cavitation including disorder, shortness and decrease of crest. The activity of SDH and content of ATP were decreased at 6 h, most serious at 3 d(P<0.01), and recovered at 7 d after radiation. The activity of ATPase and MAO increased notably at 1 d and 3 d after radiation(P<0.01). Conclusions Microwave can damage the structure and function of mitochondria in rat hippocampus, and cause the energy metabolism of enzyme disorder. |
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