| 刘勇,王登高,张广斌,周文,杨学森.微波辐射对家兔小脑运动性学习记忆和GluR2蛋白质磷酸化的影响[J].中华放射医学与防护杂志,2007,27(6):599-601 |
| 微波辐射对家兔小脑运动性学习记忆和GluR2蛋白质磷酸化的影响 |
| Effects of microwave exposure on motor learning and GluR2 phosphorylation in rabbit cerebellum |
| 投稿时间：2006-11-16 |
| DOI： |
| 中文关键词: 微波辐射 小脑 运动性学习 谷氨酸受体2(GluR2) 蛋白质磷酸化 |
| 英文关键词:Microwave exposure Cerebellum Motor learning GluR2 Phosphorylation |
| 基金项目:国家自然科学基金资助项目(30170237) |
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| 中文摘要: |
| 目的 观察微波辐射对家兔小脑运动性学习记忆功能的损伤效应,检测微波辐射对谷氨酸受体2(GluR2)磷酸化的影响。方法 选择建立牢固瞬膜条件反射的家兔,随机分为对照组和微波辐射组(辐射后0、3、24和72 h等4个时相点),辐射组给予平均功率密度90 mW/cm2的微波辐射30 min,测定辐射后家兔的即刻肛温并计算比吸收率(SAR)值;检测辐射后即刻家兔瞬膜条件反射的变化;蛋白质免疫印迹法检测家兔小脑GluR2及其磷酸化蛋白质的表达变化。结果 90 mW/cm2微波辐射后即刻家兔肛温显著升高3.02℃,SAR值为8.74 W/kg;瞬膜条件反射在辐射后0 h显著降低,3 h后基本恢复至正常水平;小脑GluR2的蛋白表达水平无显著变化但GluR2的磷酸化水平在微波辐射后即刻显著降低。结论 90 mW/cm2的微波辐射30 min能使家兔产生明显热效应,并影响小脑GluR2的磷酸化,最终导致小脑运动性学习记忆功能障碍。 |
| 英文摘要: |
| Objective To investigate the effects of microwave exposure on motor learning and Glutamate receptor 2(GluR2) phosphorylation in rat cerebellum. Methods The rabbits were trained for seven days to form eye_blink conditioning,and then divided randomly into control and microwave exposure group (at hours 0,3,24 and 72 subgroups after exposure,respectively). The rabbits were accepted 90 mW/cm2 microwave exposure for 30 minutes, and the rectal temperature were detected immediately after exposure and specific absorption rate (SAR) value were calculated. Eye-blink conditioning were detected immediately after exposure, and cerebellar GluR2 protein and GluR2 phosphorylation were detected with Western blotting. Results Rectal temperature of rabbits were increased by 3.02℃ after exposure, and SAR value was 8.74 W/kg. The eye_blink conditioning decreased significantly after exposure, and cerebellar GluR2 protein expression had no significant alteration but phosphorylation reduced significantly after exposure. Conclusions 90 mW/cm2microwave exposure has injurious effects on cerebellar GluR2 phosphorylation and motor learning. |
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