| 郑辉,甄荣,廖京辉,等.辐射损伤后不同阶段细胞内外一氧化氮和总抗氧化能力变化的实验观察[J].中华放射医学与防护杂志,2007,27(6):546-548.ZHENG Hui,ZHEN Rong,LIAO Jing-hui,et al.Changes in nitric oxide content, anti_superoxide anion radicals and total ability of anti_oxidize in rat after distinct stage of irradiation[J].Chin J Radiol Med Prot,2007,27(6):546-548 |
| 辐射损伤后不同阶段细胞内外一氧化氮和总抗氧化能力变化的实验观察 |
| Changes in nitric oxide content, anti_superoxide anion radicals and total ability of anti_oxidize in rat after distinct stage of irradiation |
| 投稿时间：2007-07-16 |
| DOI： |
| 中文关键词: 电离辐射损伤 一氧化氮 抗超氧阴离子自由基 总抗氧化能力 |
| 英文关键词:Irradiation damage Nitric oxide Anti-superoxide anion radicals Total ability of anti-oxidization |
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| 中文摘要: |
| 目的 观察不同受照剂量辐射损伤对细胞内外一氧化氮(NO)含量、抗超氧阴离子自由基活力和总抗氧化能力变化的影响。方法 采用60Co γ射线照射对CD大鼠进行0.4、0.8、1.6、3.2和6.4 Gy照射,制作辐射损伤模型。对不同阶段受照组大鼠细胞内外NO、抗超氧阴离子自由基和总抗氧化能力进行检测。结果 1 受照1 d后细胞内外NO含量较正常对照组明显增高,其增高幅度与受照剂量有关。照射21 d后细胞内外NO含量均较照射1 d明显降低,但受照剂量大于0.8 Gy,NO含量仍高于对照组水平。2 辐射损伤对机体内自由基防御能力有明显的破坏作用,其破坏程度与照射剂量有关。低剂量照射后被破坏的自由基防御系统可逐渐恢复,但照射剂量大于1.6 Gy,其恢复较慢。照射剂量大于3.2 Gy,受照动物发生死亡而无法恢复。结论 电离辐射可诱发细胞内外抗超氧阴离子自由基活力和总抗氧化能力损伤和NO含量增加,其变化幅度与受照剂量有密切的关系。辐射损伤恢复期上述改变可明显改善。 |
| 英文摘要: |
| Objective To investigate the changes in nitric oxide (NO) content, anti-superoxide anion radicals and total ability of anti-oxidize in rat after iradiation damage.Methods The five groups rats were irradiated with 60Co gamma rays (0.4,0.8,1.6,3.2 and 6.4 Gy). The NO content, anti-superoxide anion radicals and total ability of anti-oxidization outside-inside cell were measured after distinct stages of irradiation.Results 1 At 1 day after irradiation,the content of NO was significantly increased outside-inside cell and the range of changes was related to the increase of irradiation dosage. At 21 days after irradiation, the content of NO was lower than that at 1 day after irradiation, but the levels of NO at dose over 0.8 Gy were still higher than that in normal group. 2 Irradiation could hurt the defensive ability of the free radicals, and the degree of harm had relation with the irradiated dose. The defensive ability of the free radicals could recover after low irradiated dose, but it recovered slowly over 1.6 Gy dose and never recovered over 3.2 Gy dose due to animal death.Conclusions Irradiation could induce the injury of anti-superoxide anion radicals and total ability of anti-oxidize, and increase the content of nitric oxide. This degree of changes was related to the irradiated dosage. In convalescence stage after irradiation, these abnormal changes could be significantly improved. |
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