| 刘英,李杨,彭瑞云,王水明,高亚兵,马俊杰,宋良文.C57BL/6J和C3H/HeN小鼠放射性肺损伤进程的比较研究[J].中华放射医学与防护杂志,2007,27(6):530-533 |
| C57BL/6J和C3H/HeN小鼠放射性肺损伤进程的比较研究 |
| A comparative study of the progression of radiation pulmonary injury in C57BL/6J and C3H/HeN mice |
| 投稿时间：2006-12-06 |
| DOI： |
| 中文关键词: 肺纤维化 C57BL/6J小鼠 C3H/HeN小鼠 胶原 |
| 英文关键词:Pulmonary fibrosis C57BL/6J mice C3H/HeN mice Collagen |
| 基金项目:国家自然科学基金资助项目(30470774) |
|
| 摘要点击次数: 2746 |
| 全文下载次数: 1718 |
| 中文摘要: |
| 目的 比较C57BL/6J和C3H/HeN两种小鼠放射性肺损伤进程的异同。方法 采用20 Gy 60Co γ射线照射C57BL/6J和C3H/HeN两种小鼠复制动物模型,应用天狼猩红染色、羟脯氨酸含量测定、免疫组织化学等方法检测Ⅰ、Ⅲ型胶原分布、羟脯氨酸含量、纤连蛋白(FN)、层粘连蛋白(LN)和α-平滑肌肌动蛋白(α-SMA)表达变化。结果 照后1、3和6个月C57BL/6J小鼠肺组织病变经历炎症期、增生期和纤维化期,胶原沉积增多,照后1和3个月 FN表达明显高于对照组(P<0.01),照后6个月逐渐减少, LN表达在照后呈渐进性增加,α-SMA表达比C3H/HeN强;照后1、3和6个月 C3H/HeN小鼠肺组织主要表现为间质性炎症改变,FN表达于照后1、3和6个月与对照组相比无明显变化,LN表达于照后1和3个月明显增强,6个月逐渐减少。结论 20 Gy 60Co γ射线照射成功复制易/抗放射性肺纤维化动物模型。C57BL/6J小鼠发生放射性肺纤维化,以胶原沉积增多出现早且显著为特征;C3H/HeN小鼠未发生放射性肺纤维化。 |
| 英文摘要: |
| Objective To compare the course of radiation pulmonary injury in C57BL/6J and C3H/HeN mice.Methods C57BL/6J and C3H/HeN mice irradiated with 20 Gy 60Co gamma rays were used as animal models. Sirius red staining and hydroxyproline measurement were used to detect the distribution of type Ⅰ and Ⅲ collagens and the content of pulmonary hydroxyproline. Immunohistochemistry was used to observe the changes of the expressions of fibronectin (FN) and laminin (LN) and alpha-smooth muscle actin(α-SMA) in the lung.Results The changes in the lungs in C57BL/6J mice were as follows: the pathological changes undergone interstitial pneumonia, proliferation and fibrosis; significant increase of collagen deposition; FN increased rapidly to a significantly higher level at 1 and 3 months after irradiation than that in the control (P<0.01), and then decreased gradually to a normal level at 6 month after irradiation; LN elevated gradually after irradiation; the expressions of α-SMA were more intense than that in C3H/HeN mice. The changes in the lungs in C3H/HeN mice were as follows: the pathological changes mainly developed institial pneumonitis; no significant changes of FN expression was observed after irradiation compared to that in the control; LN increased significantly at 1 and 3 months after irradiation, and then decreased gradually.Conclusions The models of radiation pulmonary fibrosis_sensitive and_resistant were established by irradiation with gamma rays, C57BL/6J mice developed late radiation pulmonary fibrosis, and were characterized by the significant accumulation of collagen. C3H/HeN mice did not develop radiation pulmonary fibrosis. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|