中华放射医学与防护杂志

张玉松,傅晋祥,周剑影,等.电离辐射联合肿瘤坏死因子相关凋亡诱导配体对乳腺癌MCF-7细胞株作用的研究[J].中华放射医学与防护杂志,2007,27(5):457-459.ZHANG Yu-song,FU Jin-xiang,ZHOU Jian-ying,et al.Effect of Tumor Necrosis factor related apoptosis-inducing ligand(TRAIL) combined with ionizing radiation on proliferation and apoptosis of breast cancer MCF-7 cell lines[J].Chin J Radiol Med Prot,2007,27(5):457-459

电离辐射联合肿瘤坏死因子相关凋亡诱导配体对乳腺癌MCF-7细胞株作用的研究

Effect of Tumor Necrosis factor related apoptosis-inducing ligand(TRAIL) combined with ionizing radiation on proliferation and apoptosis of breast cancer MCF-7 cell lines

投稿时间：2006-10-10

DOI：

中文关键词: 乳腺癌肿瘤坏死因子相关凋亡诱导配体电离辐射

英文关键词:Breast cancer TRAIL Ionizing radiation

基金项目:

作者	单位	E-mail
张玉松	215004 苏州大学附属第二医院肿瘤职业病科	zhangyusongcn@yahoo.com.cn
傅晋祥	215004 苏州大学附属第二医院肿瘤职业病科
周剑影	215004 苏州大学附属第二医院肿瘤职业病科
周丽英	215004 苏州大学附属第二医院肿瘤职业病科
郭晓葵	215004 苏州大学附属第二医院肿瘤职业病科
庄志祥	215004 苏州大学附属第二医院肿瘤职业病科

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中文摘要:

目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对人乳腺癌MCF-7细胞株的作用以及与放射治疗联合的可行性。方法 1×10⁴/ml MCF-7细胞接种至96孔板培养后加入不同浓度的TRAIL、给予不同剂量照射及TRAIL与电离辐射联合,MTT法检测细胞抑制率。MCF-7细胞接种至6孔板培养后,分别加入TRAIL(1 μg/ml)、给予8 Gy照射及TRAIL与8 Gy照射联合,流式细胞术检测不同处理组细胞的凋亡率。应用RT-PCR法检测经不同处理后的细胞凋亡相关基因的表达情况。结果 TRAIL(1 μg/ml)与4、8和12 Gy照射联合后对细胞的抑制率明显增加,与单独应用TRAIL及单独照射相比,差异有统计学意义。RT-PCR结果显示Bcl-2、Bcl-Xl基因在TRAIL与8 Gy照射联合时表达减弱。结论在体外实验中,乳腺癌MCF-7细胞株对TRAIL不敏感,但TRAIL与电离辐射照射联合后抗肿瘤作用增强,TRAIL可能是一种有临床应用潜能的新型抗肿瘤生物制剂。

英文摘要:

Objective To investigate the effect of Tumor necrosis factor related apoptosis-inducing ligand (TRAIL) on breast cancer MCF-7 cell lines and the possibility of TRAIL combined with radiotherapy. Methods 1×10⁴/ml MCF-7 cell suspension were added to each well of 96-well plates, MCF cell were treated with radiotherapy(RT), TRAIL at different concentration or RT combined with TRAIL. MTT working solution was added and calculated the inhibitory rates of MCF-7 cells. MCF-7 cell suspension was added to 6-well plates then treated with TRAIL(1 μg/ml), 8 Gy RT or TRAIL combined with 8 Gy RT. The rates of apoptosis were detected by flow cytometry after incubated 48 h. RT-PCR methods were employed to analysize the expression of apoptosis related gene in different treatment group. Results MCF-7 cell lines were resistant to TRAIL,but the inhibitory rate was upregulated when MCF-7 cell was treated with TRAIL combined with RT, which had a significant difference compared with RT or TRAIL alone. The expression of Bcl-2 and Bcl-Xl gene were down_regulated when MCF-7 cell lines was treated with 8 Gy RT combined with TRAIL. Conclusions In vitro, MCF-7 cell lines are resistant to TRAIL, but TRAIL combined with radiotherapy increased the cytotoxic effect. TRAIL has a promising prospect in clinical use.

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