| 张克君,李德春,朱东明,等.重组腺病毒mIκBα基因联合γ射线治疗肝细胞癌的实验观察[J].中华放射医学与防护杂志,2007,27(5):454-457.ZHANG Ke-jun,LI De-chun,ZHU Dong-Ming,et al.Experimental study on the effects of recombimant adenoviral-mediated mIκBα gene combined with irradiation on the treatment of hepatocarcinoma[J].Chin J Radiol Med Prot,2007,27(5):454-457 |
| 重组腺病毒mIκBα基因联合γ射线治疗肝细胞癌的实验观察 |
| Experimental study on the effects of recombimant adenoviral-mediated mIκBα gene combined with irradiation on the treatment of hepatocarcinoma |
| 投稿时间：2006-10-16 |
| DOI： |
| 中文关键词: 重组腺病毒mIκBα基因 基因治疗 放射治疗 肝细胞癌 |
| 英文关键词:AdmIκBα gene Gene therapy Radiotherapy Hepatocarcinoma |
| 基金项目: |
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| 摘要点击次数: 5120 |
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| 中文摘要: |
| 目的 探讨重组腺病毒mIκBα基因(AdmIκBα)联合γ射线对人高转移性肝细胞癌HCC9204细胞和裸鼠HCC9204移植瘤生长的影响。 方法 利用有限稀释法测定病毒滴度,以不同感染复数(MOI)的Ad IκBαm(10、20、30、50)转染HCC9204细胞,Western blotting法检测转染前后细胞P65表达和mIκBα表达。采用4 Gy γ射线对各组细胞进行治疗。MTT法检测转染组、转染组+放射组、对照组细胞生长抑制率。建立裸鼠HCC9204肝癌动物模型,肿瘤局部注射AdmIκBα 30 MOI并联合6 Gy γ射线治疗,在不同时间测量肿瘤体积。 结果 病毒滴度1.252×109pfu/ml。mIκBα蛋白随AdmIκBα剂量增加而进行性增加, P65蛋白随AdmIκBα剂量的增加呈进行性递减。转染组体外细胞生长随AdmIκBα剂量的增加逐渐受抑制,经γ射线照射后生长抑制更加明显。AdmIκBα转染和放射联合治疗后7~28 d,裸鼠肿瘤生长速率明显低于单纯照射组、单纯转染治疗组。 结论 AdmIκBα转染联合γ射线治疗可增强对肝癌细胞杀灭作用,联合治疗明显优于单纯放疗组和单纯基因治疗组。 |
| 英文摘要: |
| Objective To explore the effect of recombinant adnenovirus vector mediated mutant IκBα (mIκBα) combined with radiation on the hepatocarcinoma. Methods Limited dilution method was used to test the virus titer in 293 cells. The HCC9204 cells were infected with MOI 10,20,30 and 50 for 48 h, respectively.The expression of p65 and mκB a protein was analyzed by Western blot. Transfected HCC9204 cells and controls were treated with 4 Gy γ rays. The inhibition rate of HCC9204 cells was examined by MTT. Rat modlles of HCC9204 was constructed. AdmIκBα plasmids were injected into tumor tissue and the tumors were administered with 6 Gy γ irradiation 48 hours later.Tumor growth at diferent time points was recorded during 28 days. Results The titer of AdmIκBα is 1.252×109 pfu/ml. The expression of mIκB protein was increased with titer of AdmIκBα, and p65 protein began to decrease when MOI was 10,and reached the lowest when MOI was 50, they were all dose-dependent. The proliferation of HCC9204 cell lines were suppressed, as was more significant combined with radiation,and the effect was in a viral dose-dependent manner.From days 7 to 28 after AdmIκBα gene and radiotherapy, the tumor growth was significantly slower than after irradiation or gene therapy alone. Conclusions Recombimant adenoviral-mediated mIκBα gene,combined with irradiation,can increase the cell-killing effect. It is better than that of either one alone. |
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