中华放射医学与防护杂志

姚雨石,潘真,郁成雨,等.siRNA抑制STAT3基因对HepG2细胞辐射敏感性的影响[J].中华放射医学与防护杂志,2007,27(5):444-446.YAO Yu-shi,PAN Zhen,YU Cheng-yu,et al.Variation of radio-sensitivity of HepG2 cells by short interferencing RNA blockage of STAT3 gene[J].Chin J Radiol Med Prot,2007,27(5):444-446

siRNA抑制STAT3基因对HepG2细胞辐射敏感性的影响

Variation of radio-sensitivity of HepG2 cells by short interferencing RNA blockage of STAT3 gene

投稿时间：2006-08-28

DOI：

中文关键词: siRNA STAT3 辐射敏感性

英文关键词:Short interferencing RNA STAT3 Radiosensitivity

基金项目:

作者	单位	E-mail
姚雨石	200433 上海, 第二军医大学海医系放射医学教研室
潘真	200433 上海, 第二军医大学海医系放射医学教研室	zhenpan2003@yaho.com.cn
郁成雨	200433 上海, 第二军医大学海医系放射医学教研室
唐古生	200433 上海, 第二军医大学海医系放射医学教研室
秦阳华	200433 上海, 第二军医大学海医系放射医学教研室
祝宝让	200433 上海, 第二军医大学海医系放射医学教研室

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中文摘要:

目的研究瞬时转染的siRNA抑制STAT3基因表达对人肝癌HepG2细胞辐射敏感性的影响。方法以人肝癌HepG2细胞作为研究对象,脂质体包裹化学合成的siRNA进行转染。采用实时RT-PCR和Western blotting法分别从mRNA水平和蛋白水平检测STAT3基因的表达及编码蛋白磷酸化活化的改变,用CCK-8法和Hoechst 33258 DNA染色法检测辐射敏感性的变化。结果 STAT3特异性的siRNA转染后HepG2细胞中STAT3基因mRNA拷贝数、STAT3蛋白表达和磷酸化水平均明显降低。mRNA水平的抑制效率可达70%。siRNA转染联合⁶⁰Co γ射线照射后HepG2细胞增殖活力显著低于对照组(P<0.05)。结论针对人STAT3基因的siRNA能够抑制HepG2细胞STAT3基因的表达,降低STAT3蛋白活化水平,进而产生辐射增敏作用。

英文摘要:

Objective To explore the influences on radiosensitivity of HepG2 cells after STAT3 gene inhibition by transiently transfected short interferencing RNA(siRNA). Methods Human hepatic cancer HepG2 cells were transfected with lipid coated siRNA. STAT3 gene expression and its encoding protein phosphoralation were analyzed by RT-PCR at mRNA level and by Western blotting at protein level, respectively. Radiosensitivity of HepG2 cells was evaluated using CCK-8 analysis and Hoechst 33258 DNA staining. Results STAT3 mRNA, STAT3 protein expression and its phosporalation level were obviously decreased after STAT3 specific siRNA transfection. The inhibition ratio at mRNA level was around 70%. In contrast with control group,viability of HepG2 cells was dramatically decreased when siRNA transfection was combined with ⁶⁰Co γ ray(P<0.05). Conclusions Human STAT3 gene targeting siRNA inhibited the expression of STAT3 gene, and consequently decreased the activation of STAT3 protein, which increased the radiosensitivity of HepG2 cells.

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