| 张萍,周志国,高献书,等.今又生联合h-R3对不同放射敏感性食管癌细胞的作用研究[J].中华放射医学与防护杂志,2007,27(5):427-429.ZHANG Ping,ZHOU Zhi-guo,GAO Xian-shu,et al.Effect of recombinant adenoviruses-p53 anticancer injection (Gendicine) combined with human monoclonal antibody to EGFR (h-R3) on esophageal cancer cells with different radiosensitivities[J].Chin J Radiol Med Prot,2007,27(5):427-429 |
| 今又生联合h-R3对不同放射敏感性食管癌细胞的作用研究 |
| Effect of recombinant adenoviruses-p53 anticancer injection (Gendicine) combined with human monoclonal antibody to EGFR (h-R3) on esophageal cancer cells with different radiosensitivities |
| 投稿时间：2006-10-18 |
| DOI： |
| 中文关键词: 食管癌细胞 重组腺病毒-p53抗癌注射液(今又生) 表皮生长因子受体 凋亡 |
| 英文关键词:Esophageal cancer cell lines Recombinant adenoviruses-p53 anticancer injection (Gendicine) Epidermis growth factor receptor Apoptosis |
| 基金项目:国家自然科学基金资助项目(30240057);河北省自然科学基金资助项目(303508) |
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| 中文摘要: |
| 目的 探讨重组腺病毒-p53抗癌注射液(今又生)、重组人源化表皮生长因子受体单克隆抗体(h-R3)单独及联合应用对不同放射敏感性食管癌细胞的作用。方法 用MTT法测定h-R3、今又生及两药联合对TE13及放射抗拒性细胞TE13R120的生长情况的影响;采用流式细胞术检测两种药物单独及联合作用对TE13、TE13R120细胞周期分布的影响及对Bax、Bcl-2蛋白表达的影响。结果 h-R3、今又生及联合用药组均对两种细胞增殖产生抑制作用,联合用药组对两种细胞的抑制作用高于h-R3单药组和今又生单药组,但差异均无统计学意义(P>0.05)。h-R3与今又生同时作用后12 h,对TE13细胞产生明显的G1期阻滞(P<0.05),而对TE13R120可产生轻度的G1期阻滞。两药同时作用后12 h,对凋亡相关蛋白Bax、Bcl-2表达的影响不明显。结论 h-R3与今又生联合应用对不同放射敏感性的食管癌细胞产生了增殖抑制作用,这种增殖抑制作用的发生可能与G1期阻滞有关,其具体机制有待于进一步研究。 |
| 英文摘要: |
| Objective To observe the effect of recombinant adenoviruses-p53 anticancer injection (Gendicine) combined with a human monoclonal antibody to EGFR (h-R3) on esophageal cancer cells with different radiosensitivities. Methods The inhibition effect of cell growth and the influence by h-R3 and Gendicine were measured with MTT method. Changes of cell cycle and expressions of Bax and Bcl-2 after treatment with h-R3 and Gendicine were detected by FCM. Results h-R3, Gendicine and every experimental group all showed the antiproliferative effect in vitro growth profile of each esophageal cell line. Inhibition rate of the simultaneous administration was higher than h-R3 or the Gendicine group. At 12 hours after exposure to h-R3 and Gendicine, FCM analysis demonstrated that h-R3 and Gendicine treatment induced accumulation of TE13 cells in G1, with statistical significance (P<0.05). While there was a slight accumulation of TE13R120 cells in G1 phase but there was no significance (P＞0.05). At 12 hours after treatment with h-R3 and Gendicine, the pro-apoptotic Bax expression was increased and anti-apoptotic Bcl-2 expression was decreased, with no significant difference (P＞0.05). Conclusions The anti-proliferation effect of h-R3 and Gendicine was observed on different radiosensitivities esophageal cancer cells,which may be concerned with accumulation of cells in G1 phase. But the mechanism needs to be studied further. |
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