| 吴春明,李伟华,刘荣耀,等.小剂量照射对脑室周围神经干细胞的影响[J].中华放射医学与防护杂志,2007,27(4):334-336.WU Chun-ming,LI Wei-hua,LIU Rong-yao,et al.Experiment study of single low dose irradiation on subventricular zone cell[J].Chin J Radiol Med Prot,2007,27(4):334-336 |
| 小剂量照射对脑室周围神经干细胞的影响 |
| Experiment study of single low dose irradiation on subventricular zone cell |
| 投稿时间：2006-06-04 |
| DOI： |
| 中文关键词: 大鼠 神经干细胞 X射线 照射 |
| 英文关键词:Rat Neural stem cells X-ray Irradiation |
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| 中文摘要: |
| 目的 研究幼鼠全脑小剂量照射对脑室周围神经干细胞的影响。方法 6周Wistar大鼠80只,随机分为对照组40只,照射组40只,用1 Gy X射线全脑照射,照射后30和60 d,分别随机取5只处死,测量体重、脑重量。照射后6 h、7、14、30和60 d,用免疫组织化学方法动态检测脑室周围nestin阳性细胞的表达。结果 尽管照射组和对照组大鼠的体重相似,但照射组大鼠脑重量显著减少。在脑室周围,照射6 h后,许多凋亡细胞出现,同时,nestin阳性细胞明显减少(对照组的69%),7 d恢复到照射前水平,14 d nestin阳性细胞达到最大值(为对照组的182%),30 d nestin阳性细胞恢复到基线水平(为对照组的96%),并且在随后的60 d没有变化。结论 单次小剂量照射对脑室周围神经干细胞的影响是可逆的,提示在临床治疗上,连续多次X射线照射也许对神经干细胞有一个不可逆的影响,从而导致脑生长迟缓或功能异常。 |
| 英文摘要: |
| Objective To study the effects of whole brain irradiation on subventricular zone cells (SVZ) in juvenile rat. Methods Six-week-old Wistar rats, whose brains are still growing, were irradiated with single dose of 1 Gy X-ray. Their body and brain weight were measured at days 30 or 60 after irradiation. The chronological changes of the SVZ were examined at 6 h, days 7, 14, 30 or 60 after irradiation by immunohistochemistry specifically to observe the neural stem cell using anti-nestin antibodies specific for these cells. Results The rate of brain weight gain of irradiated rats significantly decreased in comparison to controls, although that of body weight gain was similar among them. Multiple apoptotic cell appeared in the SVZ at 6 h after irradiation with simultaneous reduction in nestin-positive cell (69% of the control). The cell levels recovered within a week, with the nestin-positive cells reaching maximal (180%) on day 14, returned to baseline levels within 30 days (96%) and remained unchanged for subsequent 60 days.Conclusions Single low-dose X-ray administration reversibly affected the levels of neural stem cells in the SVZ region. The result suggest that continuous multiple administration of X-ray in clinical treatment may induce irreversible changes on neural stem cells, and cause brain growth retardation or dysfunction. |
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