| 张力元,杨顺,张明芝,等.低剂量照射对小鼠骨髓移植造血功能的影响[J].中华放射医学与防护杂志,2007,27(4):331-333.ZHANG Li-yuan,YANG Shun,ZHANG Ming-zhi,et al.Experimental study of low dose radiation stimulate the haematogenesis reconstitution of the recipient after bone marrow transplantation in mice[J].Chin J Radiol Med Prot,2007,27(4):331-333 |
| 低剂量照射对小鼠骨髓移植造血功能的影响 |
| Experimental study of low dose radiation stimulate the haematogenesis reconstitution of the recipient after bone marrow transplantation in mice |
| 投稿时间：2006-06-07 |
| DOI： |
| 中文关键词: 低剂量辐射 兴奋效应 小鼠 骨髓移植 |
| 英文关键词:Low dose radiation Hormesis Mice Bone marrow transplantation |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨低剂量照射促进小鼠骨髓移植后受体造血功能的重建。方法 通过对小鼠体外骨髓细胞进行不同剂量的照射,用3H-TdR掺入法确定产生最佳刺激增殖效应的照射剂量。在骨髓移植前,对供体小鼠骨髓细胞给予最佳刺激剂量的照射,然后将被照射的骨髓细胞输入受体小鼠内,最后动态监测受体小鼠的外周血细胞和骨髓单个核细胞数量。结果 在离体情况下,经6和8 cGy低剂量照射的小鼠骨髓细胞增殖能力明显增强。用低剂量照射的骨髓细胞进行骨髓移植后,受体小鼠骨髓单个核细胞数和外周血细胞计数普遍高于相应的对照组。结论 低剂量照射可能促进小鼠骨髓移植后受体造血功能的重建。 |
| 英文摘要: |
| Objective To investigate if low dose radiation can stimulate the haematogenesis reconstitution of the recipient after bone marrow transplantation in mice.Methods Bone marrow cells were irradiated in vitro by different low dose radiation and then cultured in vitro. 3H-TdR incorporation was used to measure the reproductive activity of cells, and then the radiation dose with the best stimulating effect was determined. The donator myeloid cells were exposed to low dose radiation before the recipient mice received bone marrow transplantation; then the irradiated myeloid cells were infused to the recipient; and lastly, the counts of peripheral blood cells (PBC) and bone marrow mononuclear cells (BMMNC) were manitored in order to observe the effect of low dose radiation on haematogenesis reconstitution of the recipient animal after bone marrow transplantation.Results The reproductive activity of the bone marrow cells irradiated by 6 and 8 cGy could be improved significantly in vitro. When the recipient mice received bone marrow transplantation of the myeloid cells after low dose radiation, the counts of BMMNC and PBC were higher than those in the control group(P<0.05).Conclusions Low dose radiation can stimulate the haematogenesis reconstitution of the recipient after bone marrow transplantation. |
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