| 廖安燕,王俊杰,赵勇,等.125I粒子照射诱导人前列腺癌细胞凋亡的实验研究[J].中华放射医学与防护杂志,2007,27(4):311-313.LIAO An-yan,WANG Jun-jie,ZHAO Yong,et al.Study on apoptosis of prostate cancer cell induced by 125I seed irradiation[J].Chin J Radiol Med Prot,2007,27(4):311-313 |
| 125I粒子照射诱导人前列腺癌细胞凋亡的实验研究 |
| Study on apoptosis of prostate cancer cell induced by 125I seed irradiation |
| 投稿时间：2007-03-08 |
| DOI： |
| 中文关键词: 125I放射性粒子 前列腺癌细胞 细胞凋亡 天冬酰胺特异酶切的半胱氨酸蛋白酶 Bcl-2 |
| 英文关键词:125I seeds Prostate cancer cell Apoptosis Caspase-3 Bcl-2 |
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| 中文摘要: |
| 目的 探讨125I粒子持续低剂量率照射诱导人前列腺癌细胞凋亡的作用机制。方法用DNA琼脂糖凝胶电泳和流式细胞仪分析125I粒子持续低剂量率照射对PC3细胞的凋亡诱导作用,检测天冬酰胺特异酶切的半胱氨酸蛋白酶Caspase-3活性来观察其对PC3细胞的凋亡诱导途径,并通过间接免疫荧光技术检测其对Bcl-2表达的影响。结果 DNA琼脂糖凝胶电泳可观察到清晰的"DNA梯度",流式细胞仪检测125I粒子持续低剂量率照射对PC3细胞具有明显的凋亡诱导作用。Caspase-3活性检测表明,Caspase-3活性无明显变化。流式细胞仪分析表明Bcl-2的表达随125I粒子剂量的增加而下降。结论 125I粒子持续低剂量率照射可诱导PC3细胞凋亡,其诱导凋亡与Bcl-2表达有关,与Caspase-3活性无关。 |
| 英文摘要: |
| Objective To explore the mechanism of apoptosis induced by 125I seed irradiation on PC3 cells.Methods Human prostate cancer cell line PC3 was treated by iradiation of 125I (2.77 cGy/h) with various dose. Agarose gel electrophoresis of DNA and flows cytometry were used to detect the apoptosis of PC3 cells and indirect immunofluorescence assay was used to detect the expression of Bcl-2.The activity of Caspase-3 was measured by Caspase Colorlmetric Assay Kits.Results Apoptosis of PC3 cells could be efficiently induced by 125I seed irradiation.The apoptotic peaks were found by flow cytometry and DNA ladder appeared on 1.8％ agarose ge1.The activity of Caspase-3 on PC3 cells treated by 125I seed irradiation was not changed significantly.Bcl-2 gene expression was down-regulated with the sample concentration increased. Conclusion 125I irradiation can induce the apoptosis of PC3 cells and the mechanism of apoptosis is related with down regulation of Bcl-2 gene expression and is not related with Caspase-3 activity. |
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