| 朱艳,黄丽芳,罗小华,等.γ射线对小鼠主动脉性腺中肾区基质细胞氨基肽酶N/CD13活性的影响[J].中华放射医学与防护杂志,2007,27(4):307-310.ZHU Yan,HUANG Li-fang,LUO Xiao-hua,et al.Effect of irradiation on APN/CD13 enzymatic activity on AGM stromal cells[J].Chin J Radiol Med Prot,2007,27(4):307-310 |
| γ射线对小鼠主动脉性腺中肾区基质细胞氨基肽酶N/CD13活性的影响 |
| Effect of irradiation on APN/CD13 enzymatic activity on AGM stromal cells |
| 投稿时间：2006-11-06 |
| DOI： |
| 中文关键词: 氨基肽酶N/CD13 主动脉性腺中肾区 基质细胞 放射损伤 酶活性 |
| 英文关键词:APN/CD13 AGM region Stromal cells Irradiation-induced injury Enzymatic activity |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨小鼠主动脉性腺中肾(AGM)区基质细胞氨基肽酶N/CD13(APN/CD13)的表达和放射损伤前后该酶活性的变化及意义。方法 采用免疫组织化学染色、RT-PCR检测 AGM区基质细胞APN/CD13的表达;60Co γ射线8.0 Gy照射细胞,在不同的时间点用分光光度计检测APN/CD13的酶活性。结果 AGM区基质细胞APN/CD13呈强阳性表达;放射损伤后酶活性呈一过性降低,继而升高,4 h达高峰,24~48 h基本恢复至照射前水平。结论 APN/CD13在AGM区基质细胞上呈强阳性表达;在放射损伤后酶活性增高,可能是促进造血功能恢复的一种代偿机制。 |
| 英文摘要: |
| Objective To investigate the expression of APN/CD13 in mice embryo AGM stromal cells and the change of its enzymatic activity before and after irradiation.Methods The expression of APN/CD13 in AGM stromal cells was assayed by RT-PCR and immunohistochemistry. The stromal cells in AGM region were irradiated with 8.0 Gy of 60Co γ-rays, and APN/CD13 enzymatic activity was measured by spectrophotometer at different time points.Results The APN/CD13 expression level was enhanced significantly in AGM stromal cells. The enzymatic activity of APN/CD13 decreased temporally post-irradiation injury, then increased to the highest level 4 hours post-irradiation, and it returned to the level of before irradiation 24 to 48 hours post-irradiation.Conclusions The expression of APN/CD13 was remarkable in AGM stromal cells. The enzyme activity of APN/CD13 was temporally enhanced after irradiation, which might be one of the compensatory mechanisms to promote the hematopoietic recovery after irradiation. |
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