| 周莉,吴丛梅,黄天华.辐射诱导pEgr-p16表达增强及其体外抑制B16黑色素瘤作用[J].中华放射医学与防护杂志,2007,27(3):235-237.ZHOU Li,WU Cong-mei,HUANG Tian-hua.Enhancement of pEgr-p16 expression induced by irradiation and its anti-tumor effect on B16 melanoma cells in vitro[J].Chin J Radiol Med Prot,2007,27(3):235-237 |
| 辐射诱导pEgr-p16表达增强及其体外抑制B16黑色素瘤作用 |
| Enhancement of pEgr-p16 expression induced by irradiation and its anti-tumor effect on B16 melanoma cells in vitro |
| 投稿时间：2006-10-09 |
| DOI： |
| 中文关键词: pEgr-p16基因 辐射 B16细胞 |
| 英文关键词:pEgr-p16 gene transfection Irradiation Melanoma B16 cells |
| 基金项目:广东省科技计划资助项目(2003C30304),汕头市重点科技计划资助项目(汕府科[2006]82号) |
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| 中文摘要: |
| 目的 研究pEgr-p16重组质粒在转染的黑色素瘤B16细胞中的辐射诱导表达特性,验证其联合放射治疗体外抑制B16细胞增殖的作用。方法 将脂质体包裹的pEgr-p16重组质粒,转染B16细胞株;采用定量PCR方法检测不同剂量X射线诱导后p16的表达和同一剂量X射线诱导下p16的表达时程,流式细胞数术检测细胞凋亡率的变化;MTT法检测pEgr-p16基因协同放射治疗后B16细胞的存活情况。结果 pEgr-p16重组质粒转染B16细胞,不同剂量X射线均可诱导p16表达增强,为假照组的3.78～6.67倍(P<0.01);2 Gy X射线照射后,p16表达随时间延长而逐渐增强,在照射后72 h达到最高值。p16基因联合放射可诱导B16细胞凋亡,凋亡率高于单纯照射组和单纯基因诱导组(P<0.05～0.01);转染pEgr-p16质粒的细胞经2 Gy X射线照射,8 d后细胞数明显低于同时间其他实验组(P<0.05～0.001)。结论 pEgr-p16基因-放射联合治疗有明显的抑制黑色素瘤B16细胞生长的作用,其作用优于单纯给予射线或基因转染。 |
| 英文摘要: |
| Objective To study the antitumour effect of irradiation combination with recombined pEgr_p16 plasmid on melanoma B16 cells.Methods pEgr-p16 plasmids were transfected into B16 cell line. Quantitative RT-PCR, flow cytometry and MTT methods were used to detect the expression of p16, cell apoptosis and inhibition effect,respectively.Results The p16 expressions in different doses X-ray irradiation group were about 3.78-6.67 times higher than that in sham_irradiation group (P<0.01). The expressions of p16 gradually increased with time after exposure to 2 Gy X-ray irradiation and reached to maximum at 72 h. Apoptosis rate in transfected p16 combined with irradiation group was higher than that in either plasmid or irradiation group alone (P<0.05-0.01). The number of pEgr-p16-transfected B16 cells exposed to 2Gy X-ray irradiation was significantly less than those in other experimental groups (P<0.05-0.001).Conclusions pEgr-p16 gene transfection combined with irradiation could suppress melanoma B16 cell growth.And the inhibition was more effective than those in gene- or irradiation-therapy alone. |
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