| 王跃珍,马胜林,张爱琴,冯建国,方冼华,孙晓江,包叶江.中肺合剂防治大鼠早期放射性肺炎的初步实验研究[J].中华放射医学与防护杂志,2007,27(3):232-234 |
| 中肺合剂防治大鼠早期放射性肺炎的初步实验研究 |
| Experimental study of the protective effects of ZhongFei Decoction on radiation-induced pneumonia in rats |
| 投稿时间：2006-07-04 |
| DOI： |
| 中文关键词: 放射性肺炎 中肺合剂 羟脯氨酸 转化生长因子β1 |
| 英文关键词:Radiation pneumonia ZhongFei Decoction Hydroxyproline Transforming growth factor beta 1 |
| 基金项目:浙江省中医药科技计划项目(2006Y024) |
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| 中文摘要: |
| 目的 应用大鼠放射性肺炎模型观察中肺合剂对早期放射性肺炎的防治效果,探讨其在放射性肺炎中的作用机制,以期为临床应用提供实验依据。方法 雌性Wistar大鼠60只,随机分为对照组(0 Gy)、照射组(30 Gy)、地塞米松(DXM)组(30 Gy+DXM)和中肺合剂组(中肺合剂+30 Gy)。单次6 MV X线30 Gy照射大鼠双肺,建立复制放射性肺炎模型。分别于2和4周时各处死5只大鼠取肺组织,进行HE染色观察肺组织病理变化,检测肺系数,用生化法测定肺羟脯氨酸含量,用免疫组化法测转化生长因子β1(TGF-β1)的表达。结果 照射组大鼠肺损伤明显,肺系数、肺羟脯氨酸含量和TGF-β1的表达较对照组明显增高(P<0.05),而中肺合剂组比照射组肺充血、出血、渗出性改变及肺泡壁增厚程度减轻,且肺系数、肺羟脯氨酸含量和TGF-β1的表达有一定程度的下降。结论 中肺合剂对大鼠早期放射性肺炎有一定程度的保护作用,其机制可能是通过抑制TGF-β1表达,使放射性肺炎及纤维化病变减轻。 |
| 英文摘要: |
| Objective To investigate the protective effect and its possible mechanism of ZhongFei Decoction on radiation-induced pneumonia in rats.Methods Single irradiation was given at two thorax of female Wistar rats with 30 Gy of 6 MV X irradiation.Sixty rats were randomly divided into the control group, radiation group, radiation plus DXM and ZhongFei Decoction plus radiation group. On days 14 and 28 after treatment, 5 rats of each group were sacrificed, and their lungs were harvested for measurement of the lung index, the difference of the histopathology change, the concentration of hydroxyproline (hyp), and expression of transforming growth factor beta1 in lungs were analyzed by HE stain, biochemical method and immunohistochemical method, respectively.Results The pathological study showed marked lung injury in the radiation group while only slight hyperemia hemorrhage, exudation and thickness of alveolar walls in the lungs of ZhongFei Decoction plus radiation group, the concentration of hydroxyproline and expression of TGF-β1 in the radiation lungs increased compared with that in the control group and reduced in the ZhongFei Decoction plus radiation group compared with that in the radiation group.Conclusions ZhongFei Decoction could have protective effects on the radiation_induced pneumonia and the mechanism of its may be related with down-regulating the expression of TGF-β1 in the irritated lung tissue. |
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