| 廖安燕,王俊杰,赵勇,王济东,庄洪卿.125I粒子持续低剂量率照射对人前列腺癌细胞的抑制作用[J].中华放射医学与防护杂志,2007,27(3):226-228 |
| 125I粒子持续低剂量率照射对人前列腺癌细胞的抑制作用 |
| The inhabitation effect of 125I seed irradiation on prostate cancer cell |
| 投稿时间：2007-01-30 |
| DOI： |
| 中文关键词: 125I放射性粒子 前列腺癌细胞 细胞增殖 细胞周期 |
| 英文关键词:125I seeds Prostate cancer cell Cell proliferation Cell cycle |
| 基金项目:国家自然科学基金资助项目(30572137) |
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| 摘要点击次数: 3123 |
| 全文下载次数: 1929 |
| 中文摘要: |
| 目的 探讨125I粒子持续低剂量率照射对人前列腺癌细胞株(PC3)增殖抑制以及对细胞周期的影响。方法 125I粒子(初始剂量率2.77 cGy/h)和60Co γ射线(吸收剂量率2.215 Gy/min)对体外培养的PC3细胞进行0、0.5、1、1.5、2、4、6和8 Gy照射,用细胞计数、锥虫蓝染色和集落形成法检测细胞增殖、细胞活力和细胞存活率的情况;用单击多靶模型拟合剂量存活曲线;用流式细胞仪检测细胞周期。结果 随着照射剂量的增加,125I粒子照射组的细胞生长比60Co γ射线组更加明显地受到抑制(P<0.05)。125I粒子照射PC3细胞D0值为2.243,Dq为0.87,N为1.618,SF2为0.5。60Co照射组PC3细胞放射生物学参数D0值为2.824,Dq为1.08,N为1.587,SF2为0.7。60Co和125I粒子的RBE比值为1.4。与空白对照组相比,125I粒子组和60Co组4Gy照射细胞24h后均出现G2期阻滞。结论 125I粒子照射能抑制人前列腺癌细胞的增殖并能使PC3细胞阻滞于G2期。 |
| 英文摘要: |
| Objective To study the effect on prostate cancer cell proliferation and cell cycle treated by continuous low dose rate 125I irradiation.Methods PC3 cell was irradiated with 125I (2.77 cGy/h) and 60Co(2.215 Gy/min) at various doses.The cellular proliferation,vitality and cell surviving rate were evaluated by cytometric methods,typan_blue stain and the colony_forming assay after radiation.The cell cycle distribution was investigated by FCM. Results\ The death rate of PC3 cell exposed to 125I was higher than that exposed to 60Co gamma rays with the increase of the irradiation dose.The parameters of radiobiology of PC3 irradiated by 125I seeds were:D0=2.243,Dq=0.87,N=1.618,SF2=0.5 whereas the parameters of 60Co were:D0=2.824,Dq=1.08,N=1.587,SF2=0.7.The RBE for 125I compared with 60Co gamma rays is 1.4.The cell cycle distribution after 4 Gy 125I or 60Co irradiation was similar,with increasing in the proportion of cells in G2 phase after 24 h.Conclusions 125I irradiation could inhibit the proliferation of PC3 and induced G2 phase arrest. |
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