| 高艳,秦树桐,张成岗.基于神经细胞活性评价高功率微波照射安全性研究[J].中华放射医学与防护杂志,2007,27(2):201-202 |
| 基于神经细胞活性评价高功率微波照射安全性研究 |
| Effects of high power microwave irradiation on cellular activities of cultured primary neurons |
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| DOI： |
| 中文关键词: 高功率微波 微波照射 神经细胞 ATP酶 细胞活性 |
| 英文关键词:High power microwave Microwave irradiation Neurons ATPase Cellular activity |
| 基金项目:国家高技术研究发展专项经费, 国家自然科学基金重大研究计划(90208017) , 国家自然科学基金资助项目(30300358) |
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| 中文摘要: |
| 目的 研究平均功率密度5 mW/cm2的高功率微波(HPM)对神经细胞活性的影响,为制定微波照射的安全标准提供参考数据。方法 出生后12 h内Wistar大鼠乳鼠皮层神经细胞常规培养7 d后,进行HPM照射。采用细胞内ATP酶活性测定法,检测HPM对原代培养神经细胞总ATP酶、钙镁ATP酶和钠钾ATP酶活性的影响,并用MTT法检测照射后细胞琥珀酸脱氢酶(SDH)活性的变化,观察时间点分别为HPM照射后1和6 h,1、3和7 d。结果 原代培养神经细胞经平均功率密度5 mW/cm2的HPM连续照射6 min后,同一时间点内HPM照射组和对照组之间细胞SDH活性和各ATP酶活性均无显著差异。结论 平均功率密度为5 mW/cm2的HPM照射对原代神经细胞的活性无显著影响,提示就神经细胞活性而言,该功率密度的HPM照射是安全的。 |
| 英文摘要: |
| Objective To evaluate the biological effects of high power microwave (HPM) irradiation on cellular activities of cultured primary neurons. Methods After the cerebral cortical neurons of newborn Wistar rats were cultured for 7 days, the cultured primary neurons were exposed to high microwave irradiation with an average power density of 5 mW/cm2. The enzymatic activity of total ATPase, Ca2+, Mg2+-ATPase and Na+, K+-ATPase were measured at 1 and 6 h, 1, 3 and 7 d after irradiation. The enzymatic activities of succinate dehydrogenase (SDH) of the cultured primary neurons were also detected at the same time by the MTT method to reveal the activity of the neurons. Results The enzymatic activities of ATPase and SDH of neurons treated with microwave exposure with the average power density of 5 mW/cm2 were not significantly changed. Conclusions The HPM exposure to neurons with an average power density of 5 mW/cm2 is biologically safety to cultured primary neurons. |
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