| 耿晓华,郭鲜花,左雅慧,王小莉,王仲文.γ射线诱发人正常肝细胞基因组不稳定性研究[J].中华放射医学与防护杂志,2007,27(2):135-137 |
| γ射线诱发人正常肝细胞基因组不稳定性研究 |
| Genomic instability induced by 60Co γ ray radiation in normal human liver cells |
| 投稿时间：2006-06-18 |
| DOI： |
| 中文关键词: 基因组不稳定性 滞后效应 二次照射 单细胞凝胶电泳 微核 |
| 英文关键词:Genomic instability Delayed effects Second irradiation Single cell gel electrophoresis Micronucleus |
| 基金项目:中国核工业集团公司基金资助项目(416050202) |
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| 中文摘要: |
| 目的 探讨电离辐射诱发的基因组不稳定性效应。方法 采用60Coγ射线照射人正常肝细胞, 检测克隆形成率和微核发生率,利用单细胞凝胶电泳(SCGE)技术检测DNA损伤情况。照射2、4、6、8和10 Gy后传代培养,在40代后各剂量组再次统一照射2 Gy,进行辐射损伤的检测。结果 首次照射后,克隆形成率随受照剂量的增大而降低。存活细胞经二次照射后,SCGE结果和微核发生率结果表明,首次照射剂量与子代二次照射后的损伤程度存在剂量效应关系。结论 γ射线不仅在肝细胞中产生直接的生物效应,而且还可以诱发产生可遗传的基因组不稳定性,使子代细胞中的突变频率增加,表现出滞后的遗传改变。二次事件的放大作用是研究基因组不稳定性的一种较好方法。 |
| 英文摘要: |
| Objective To explore the genomic instability induced by 60Coγ rays. Methods The cloning efficiency and micronucleus efficiency of normal human liver cell irradiated by 60Co γ rays were detected, and the method of single cell gel electrophoresis (SCGE) was carried out to measure DNA chains damage. The fast-growing cells were divided into different dose-groups and then irradiated by 60Coγ rays. After 40 populations doubling, the progenies were secondly irradiated with 2 Gy 60Coγ rays. Results The cloning efficiency decreased with the increase of doses after the initial irradiation. After the survival cells were given second irradiation, both results of SCGE and micronucleus frequency showed that the second damage was correlated with the original irradiation doses. Conclusions 60Coγ rays can not only induce the immediate biological effects in liver cells, but also lead to the genomic instability in the descendants that leads to an enhanced frequency of genetic changes occurring among the progeny of the original irradiated cell. The expanding effect of second event helps to study the genomic instability. |
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