朱小东,曲颂,黎丹戎,曹骥,张玮.Ku蛋白表达与鼻咽癌细胞放射敏感性 关系初探[J].中华放射医学与防护杂志,2007,27(2):128-131 |
Ku蛋白表达与鼻咽癌细胞放射敏感性 关系初探 |
Relationship between the expression of Ku gene and radiosensitivity in nasopharyngeal carcinoma cell strains |
投稿时间:2006-03-17 |
DOI: |
中文关键词: 鼻咽癌细胞系 Ku蛋白 放射敏感性 |
英文关键词:Nasophryngeal carcinoma cell lines Ku gene Radiosensitivity |
基金项目:广西自然科学基金资助项目(0575090) |
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中文摘要: |
目的 探讨不同放射敏感性鼻咽癌细胞株CNE-1(鼻咽高分化鳞癌细胞株)、CNE-2(鼻咽低分化鳞癌细胞株)中DNA依赖蛋白激酶(DNA-PK)的调节亚基Ku70、Ku80基因的表达与鼻咽癌细胞放射敏感性的关系。方法 通过克隆形成实验测定CNE-1、CNE-2不同剂量的存活分数,并用线性二次模型拟合剂量存活曲线求出放射生物学参数α、β、SF2、MID值,以及四氮唑蓝比色分析法(MTT法)检测60Coγ射线4Gy照射后的细胞群体生长情况,以评价两株细胞的放射敏感性。逆转录实时荧光定量PCR技术(RT-FQ-PCR)检测照射前、后不同时间及不同剂量CNE-1、CNE-2细胞mRNA水平Ku70、Ku80基因的定量表达。结果 CNE-1在各个剂量点的存活分数均比CNE-2高,MID值分别为2.78、1.61,SF2值分别为0.627、0.341;4Gy照射后的存活率分别为88.2%、72.3%;RT-FQ-PCR显示两株细胞中均有Ku70、Ku80基因的表达,其相对表达量之比分别为1.76±0.54(P=0.07)、13.82±3.78(P=0.004),Ku80基因在CNE-2细胞中存在剂量依赖关系。结论 实验验证了CNE-2比CNE-1对射线更敏感,Ku80基因的表达与鼻咽癌细胞的放射敏感性有关。 |
英文摘要: |
Objective To elucidate the relationship between expression of Ku70/Ku80 gene (a modulating subunit of DNA-PK,which is an important component in NHEJ pathway) and radiosensitivity of nasopharyngeal carcinoma cell strains. Methods Radiosensitivity parameters of nasophryngeal carcinoma cell strains were obtained by the colony forming assay and dose-survival curve was developed according to linear quadratic model. The two cell strains were exposed to 4 Gy of 60Coγ rays, the growth rate of cell group was obtained by MTT assay, and the different radiosensitivity of two cell strains was evaluated. The dynamical changes of the expression of Ku70 and Ku80 in mRNA level in CNE-1 and CNE-2 were detected by RT-FQ-PCR at different doses. Results The survival rates of CNE-1 were higher than those of CNE-2 at each dose point. The mean inactivation dose was higher in CNE-1(2.78) than that in CNE-2(1.61),and the values of SF2 was 0.627 and 0.341, respectively. After 4 Gy irradiation, the survival rate in CNE-1 was also higher than that in the CNE-2. The ratio of relative expression of Ku70 and Ku80 in the two cell strains was 1.76±0.54(P=0.07)and 13.82±3.78(P=0.004),respectively. The expression of Ku80 in CNE-2 was correlated with the irradiation dose. Conclusions CNE-2 cells are more radiosensitive than CNE-1. The expression of Ku80 gene is correlated with the radiosensitivity of nasophryngeal carcinoma cells. |
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