| 靳巍,崔玉芳,安晓霞,等.γ射线对小鼠Th1和Th2细胞功能亚群的影响[J].中华放射医学与防护杂志,2007,27(2):124-127.JIN Wei,CUI Yu-fang,AN Xiao-xia,et al.Effects of γ-rays on Th1 and Th2 immune function of mice[J].Chin J Radiol Med Prot,2007,27(2):124-127 |
| γ射线对小鼠Th1和Th2细胞功能亚群的影响 |
| Effects of γ-rays on Th1 and Th2 immune function of mice |
| 投稿时间：2006-04-25 |
| DOI： |
| 中文关键词: Th1 Th2 γ射线 小鼠 流式细胞术 |
| 英文关键词:Th1 Th2 γ-rays mice Flow cytometry |
| 基金项目:总后“十五”基金资助项目(01MA075 ,02M010);总后“十一五”基金资助项目(06Z064) |
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| 中文摘要: |
| 目的 观察γ射线照射对小鼠辅助性T细胞等功能亚群的影响,探讨辐射所致的免疫系统损伤在细胞学和分子水平上的机制。方法 应用细胞表面标志和细胞内因子的流式细胞仪分析方法,检测小鼠外周血和脾脏Th1和Th2等淋巴细胞亚群的变化。结果 1 6 Gy γ射线照射后1d,即可见小鼠脾脏不同细胞亚群的降低,尤以CD19+和CD8+的降低更为明显,分别为对照组的30%和41%(P<0.01),照后7和14 d降至最低,分别为对照组的7.9%和14%,照后21 d开始恢复。2 对Th1和Th2功能亚群的分析表明,照后1 d,小鼠外周血和脾脏Th1亚群均明显降低,分别为对照组的2.6%和7.6%(P<0.01),而Th2亚群未见明显降低甚至在外周血出现一定程度的升高。3 由于CD8+ 亚群的显著降低,导致CD4+/CD8+ 比值的明显升高(照后1d,P<0.01),照后21 d仍未恢复到对照组水平;由于Th1亚群的显著降低,使得照射小鼠外周血和脾脏的Th2/Th1比例失衡,即发生明显的Th1/Th2模式向Th2免疫反应漂移的现象。结论 6 Gy γ射线照射后,Th1和Th2功能亚群的失衡导致小鼠免疫功能的抑制,揭示了Th1和Th2功能亚群的失衡在辐射所致的免疫损伤中具有较重要作用,为深入探索辐射损伤的分子免疫学机制提供了新的启示。 |
| 英文摘要: |
| Objective To observe the effects of 6 Gy whole body γ-irradiation on immune function of Th1 and Th2 in mouse, and to investigate the cellular and molecular mechanism of immune system injury induced by irradiation. Methods Surface marker and intracellular cytokines of lymphocytes were stained with fluorescence-labeled monoclonal antibodies, then the changes of lymphocyte subpopulations, especially the Th1 and Th2 in mouse peripheral blood and spleen were analyzed by flow cytometry. Results (1) 1 d after 6 Gy γ-irradiation, lymphocytic subsets of CD19+ and CD8+ in spleen decreased apparently and the percentages of them were only 30% and 41% of control groups respectively(P<0.01). The percentages reached the minimum at 7 d for CD19+ and 14d for CD8+ respectively,however, up to 21d post-irradiation they still did not return to control level. (2) Th1 subpopulations in mouse peripheral blood and spleen were significantly reduced at 1 d after irradiation and were only 2.6% and 7.6% of control groups(P<0.01), while Th2 cells were not decreased and instead increased to some extent in peripheral blood at 7 d post-irradiation. (3) It was found the ratio of CD4+/CD8+were significantly increased at 1 d post-irradiation in mouse spleen because of swift reduction of CD8+cells. Interestingly, either in peripheral blood or in spleen in irradiated mice, the ratio of Th1/Th2 were evidently raised because of the decrement of Th1 cells, exhibited obviously a phenomenon of predominant immune response of Th2 cells. Conclusions It is suggested that the depression of mouse immune function induced by 6 Gy γ-irradiation might be caused by changes of CD4+/CD8+ ratio, especially the imbalance of Th1/Th2 function subpopulations. It is shown that the imbalance of Th1/Th2 function subpopulations plays an important role in radiation-induced immune injury, thus providing a better insight into the molecular mechanism and new strategies for prevent and treatment measures of immune injury by irradiation. |
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