| 徐瑞明,陈光伟,武宁,等.X射线全身照射诱导小鼠脾细胞LAMP-1 表达的时程变化[J].中华放射医学与防护杂志,2007,27(2):121-123.XU Rui-ming,WU Ning,et al.Change of time-course in expression of LAMP-1 on the splenocytes after WBI of mice with X rays[J].Chin J Radiol Med Prot,2007,27(2):121-123 |
| X射线全身照射诱导小鼠脾细胞LAMP-1 表达的时程变化 |
| Change of time-course in expression of LAMP-1 on the splenocytes after WBI of mice with X rays |
| 投稿时间：2006-04-17 |
| DOI： |
| 中文关键词: X射线 T细胞 NK细胞 LAMP-1 流式细胞术 |
| 英文关键词:X rays T cells NK cells LAMP-1 Flow cytometry |
| 基金项目:国家自然科学基金资助项目(30100033) |
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| 中文摘要: |
| 目的 观察X射线全身照射对小鼠脾细胞LAMP-1表达的影响。方法 采用流式细胞术检测0.075和2 Gy X照射后不同时间(0、2、4、8、16、24和48 h)以及用Con A刺激4 h后,脾细胞表面表达LAMP-1的阳性细胞数。结果 脾细胞表面的LAMP-1在2 Gy X射线全身照射后8、16和24 h表达明显下降;0.075 Gy X射线全身照射后2 h显著升高,而48 h又下降到低于对照的水平。0.075和2 Gy X射线全身照射后再加用Con A(10 μg/μl)刺激4 h,LAMP-1的表达均增强;但是此时2 Gy的效应比0.075 Gy更明显。结论 LAMP-1参与电离辐射作用下免疫信号的传导,高剂量X射线抑制它的表达,而低剂量X射线在早期对其表达有促进作用,与体内免疫细胞的活性密切相关。Con A促进免疫细胞表面LAMP-1的表达。 |
| 英文摘要: |
| Objective To study the effect of WBI with X rays on the expression of LAMP-1 in the splenocytes of mice. Methods The LAMP-1 positive cells from mouse splenocytes were detected with flow cytometry (FCM) at different times(0,2,4,8,16,24 and 48 h) after WBI with X-rays of 0\^075 Gy and 2.0 Gy and after WBI added by Con A stimulation. Results In view of time-course effect,the LAMP-1 expression in the splenocytes was significantly inhibited at 8,16, 24 h after WBI by 2.0 Gy X-rays while the LAMP-1 expression was markedly enhanced at 2 h and suppressed at 48 h after WBI by 0.075 Gy X-rays. Compared with control group,the LAMP-1 expression in the splenocytes was significantly up-regulated with Con A stimulation(10 μg/μl) after WBI with 0.075 and 2.0 Gy X rays. With Con A added, the effect of 2.0 Gy X rays was greater than that of 0.075 Gy. Conclusions LAMP-1 plays a role in the transduction of immune signals induced by ionizing radiation and is closely correlated with the activity of immune cells. The high dose irradiation inhibits the LAMP-1 expression while the low dose likely causes the opposite biological effect in the early time. Con A enhances the expression of LAMP-1 on the surface of immune cells. |
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