| 陶思,孙汉英,刘文励.造血干细胞联合AGM基质细胞移植对骨髓移植小鼠造血重建的影响[J].中华放射医学与防护杂志,2007,27(2):117-120.TAO Si,SUN Han-ying,LIU Wen-li.Effect of cotransplantation of hematopoietic stem cells and embryonic AGM stromal cells on hematopoietic reconstitution in mice after bone marrow transplantation\[J].Chin J Radiol Med Prot,2007,27(2):117-120 |
| 造血干细胞联合AGM基质细胞移植对骨髓移植小鼠造血重建的影响 |
| Effect of cotransplantation of hematopoietic stem cells and embryonic AGM stromal cells on hematopoietic reconstitution in mice after bone marrow transplantation\ |
| 投稿时间：2006-02-28 |
| DOI： |
| 中文关键词: 主动脉-性腺-中肾区 同基因骨髓移植 造血重建 造血微环境 |
| 英文关键词:Aorta-gonad-mesonephros (AGM) region Syngeneic bone marrow transplantation Hematopoietic reconstitution Hematopoietic microenvironment |
| 基金项目:国家自然科学基金资助项目(C03030202) |
|
| 摘要点击次数: 4506 |
| 全文下载次数: 2152 |
| 中文摘要: |
| 目的 探讨造血干细胞与主动脉-性腺-中肾(aorta-gonad-mesonephros,AGM)区来源的基质细胞联合移植对同基因骨髓移植(bone marrow transplantation, BMT)小鼠BMT后骨髓造血的影响。方法 建立同基因骨髓移植小鼠模型,随机分成4组:空白对照组、BMT组、BMT联合AGM基质细胞移植组(联合移植组)及川芎嗪组,另设正常组。分别于BMT后7、14、21和28 d检测外周血细胞、骨髓单个核细胞(bone marrow mono-nuclear cells,BMMNC),3、7、10、14、21和28 d检测骨髓组织学变化。结果 联合移植组骨髓单个核细胞较川芎嗪组恢复快,联合移植组及川芎嗪组外周血血细胞、骨髓单个核细胞、骨髓组织恢复均较单纯BMT组快,差异有统计学意义。结论 BMT联合AGM基质细胞移植对骨髓移植造血重建具有促进作用。 |
| 英文摘要: |
| Objective To explore the effects of cotransplantation of hematopoietic stem cells and stromal cells derived from aorta-gonad-mesonephros (AGM) region on hematopoietic reconstitution in mice after bone marrow transplantation (BMT). Methods The typical mice model of syngeneic BMT was established and the mice were randomly divided into 4 groups: the control group, the BMT group, the group of cotransplantation of HSC with AGM stromal cells (the cotransplantation group) and the ligustrazine group (the LT group). On days 3, 7, 10, 14, 21 and 28 after BMT, the peripheral blood cells and bone marrow mononuclear cells (BMMNC) were counted, and histology changes of bone marrow were detected. Results The levels of peripheral WBC, RBC, platelet, and BMMNC in the contransplantation group were significantly higher than those in the single BMT group and the LT group (P<0.05). Conclusions Cotransplantation with AGM stromal cells could significantly promote hematopoietic reconstruction in mice after BMT. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|