| 魏巍,李文建,苏旭,金晓东,郭传玲.不同LET射线对SMMC-7721细胞辐射敏感性与周期的影响[J].中华放射医学与防护杂志,2007,27(1):53-56 |
| 不同LET射线对SMMC-7721细胞辐射敏感性与周期的影响 |
| Analysis of radiosensitivity and cell cycle of SMMC-7721 hepatoma cell line irradiated with different LET rays |
| 投稿时间：2006-02-28 |
| DOI： |
| 中文关键词: 电离辐射 SMMC-7721细胞 辐射敏感性 细胞周期 细胞凋亡 |
| 英文关键词:Ionizing radiation SMMC-7721 cell line Radiosensitivity Cell cycle Cell apoptosis |
| 基金项目:国家自然科学基金资助项目(10335050);科技部重大基础研究前期研究专项资助项目(2003CCB00200) |
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| 中文摘要: |
| 目的 研究不同LET射线对SMMC-7721肝癌细胞辐射敏感性、周期进程和凋亡的影响,为重离子治疗癌症的临床应用积累基础数据。方法 以0、0.5、 1、 2、 4和8 Gy的12C6+离子及X射线分别照射处于指数生长期的SMMC-7721细胞,用克隆形成率测定细胞辐射敏感性,通过流式细胞术测定细胞DNA含量以确定各时相细胞的比例及细胞凋亡情况。结果 12C6+离子所致的SMMC-7721细胞存活率明显低于X射线。随着吸收剂量的增加和修复时间的延长,12C6+离子能导致更显著的细胞S期阻滞、G2/M期阻滞延迟和细胞凋亡。结论 与X射线相比,12C6+离子辐照能更有效地杀伤HepG2肝癌细胞,并诱导其凋亡。 |
| 英文摘要: |
| Objective To study the effect of different LET rays on change of radiosensitivity, cell cycle and apoptosis in SMMC-7721 hepatoma cells. Methods Exponentially grown SMMC-7721 cells were irradiated with 12C6+ ions and X-ray at doses of 0, 0.5, 1, 2, 4 and 8 Gy. The radiosensitivity of cells was assessed by means of the colony-forming assay. The DNA content, percentage of each cell-cycle phase and the apoptosis rate were determined with flow cytometry analysis. Results The survival fraction of SMMC-7721 hepatoma cells was remarkably lower after irradiation with 12C6+ ions than that of X-ray. With the increases of dose and repair time, the S phase arrest, G2/M phase arrest delay and the apoptosis in SMMC-7721 cells were significantly induced by 12C6+ ions. Conclusions 12C6+ ions can kill the SMMC-7721 cells and induce apoptosis more effectively in the cells than X-rays. |
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