| 陈英,刘秀林,姚波,骆亿生,李友.山东济宁辐射事故受照人员生物剂量估算及彗星电泳检测分析[J].中华放射医学与防护杂志,2007,27(1):22-24 |
| 山东济宁辐射事故受照人员生物剂量估算及彗星电泳检测分析 |
| Biological dose estimation and Comet analysis of the victims in a high dose 60Co radiation accident |
| 投稿时间：2006-01-16 |
| DOI： |
| 中文关键词: 大剂量照射 染色体畸变 生物剂量 彗星电泳 |
| 英文关键词:High dose irradiation Chromosome aberrations Biological dose Comet assay |
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| 中文摘要: |
| 目的 探索特大剂量照射后外周血和骨髓染色体培养方法,拟合6 Gy以上大剂量照射染色体双着丝点+环剂量-效应曲线,对山东济宁"10.21"事故受照者进行准确生物剂量估算和DNA损伤检测。方法 采集2例受照者外周血和骨髓细胞,制备染色体标本,计数双(多)着丝点+环数目;用正常离体人血拟合6～22 Gy双+环剂量效应曲线及数学方程;对2例事故受照者进行生物剂量估算。用碱性单细胞凝胶电泳方法检测受照者外周血DNA损伤。 结果 B的外周血染色体双+环平均数为4.47个/细胞;A的外周血培养无分裂细胞,骨髓染色体双+环平均数为9.15个/细胞。用6～22 Gy剂量效应方程估算全身平均受照剂量,B为9.4 Gy,A为19.5 Gy。单细胞凝胶电泳可见2例受照者的多数彗星细胞呈小头大尾形状。结论 用新建立的6～22 Gy染色体畸变剂量效应曲线估算2例受照者的生物剂量,已分别达到极重度骨髓型放射病和肠型放射病水平。 |
| 英文摘要: |
| Objective To explore the methods of chromosome preparation in human peripheral blood and bone marrow after very high dose exposure and fit the dose-response curve of dicentrics and rings in the range of high doses over 6 Gy for estimating biological dose and detecting DNA damage in the victims of "10.21" accident. Methods The samples of peripheral blood and bone marrow in 2 victims were collected to prepare chromosome mataphases and dicentrics (multicentrics) + rings were counted. The dose-response curve and equation of human blood irradiated between 6-22 Gy in vitro were established and applied to assess biological dose of 2 victims. In addition, their DNA damages were tested by alkaline single cell gel electrophoresis. Results The dicentric+ring numbers of 4.47 per cell in victims B's peripheral blood lymphocytes and 9.15 per cell in victim A's bone marrow who had no mitosis in peripheral blood cell. The whole body average doses of victims B and A estimated by 6-22 Gy equation arrived at 9.4 Gy and 19.5 Gy, respectively. The serious DNA damages were expressed by small head and large tail comet figures. Conclusions The biological doses of 2 victims estimated by 6-22 Gy dose-response curve have reached the levels of extreme grave bone marrow and intestinal ARS, respectively. |
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