| 沈波,鞠桂芝,刘扬,等.X射线诱导survivin在EL-4细胞中的表达及与P53表达的相互关系[J].中华放射医学与防护杂志,2006,26(6):563-565.SHEN Bo,JU Gui-zhi,LIU Yang,et al.Expressions of survivin and P53 and their correlation in EL-4 cell line[J].Chin J Radiol Med Prot,2006,26(6):563-565 |
| X射线诱导survivin在EL-4细胞中的表达及与P53表达的相互关系 |
| Expressions of survivin and P53 and their correlation in EL-4 cell line |
| 投稿时间：2005-12-13 |
| DOI： |
| 中文关键词: survivin P53 细胞凋亡 |
| 英文关键词:Survivin P53 Apoptosis cell |
| 基金项目:国家自然科学基金资助项目(30270346) |
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| 摘要点击次数: 3732 |
| 全文下载次数: 1908 |
| 中文摘要: |
| 目的 观察电离辐射对EL-4细胞survivin蛋白表达的影响及与P53表达的关系。方法 采用流式细胞术(FCM)观察EL-4细胞接受4GyX射线照射后,survivin蛋白的表达、P53表达及细胞凋亡的变化。结果 4GyX射线照射EL-4细胞,Survivin蛋白表达受抑制,在4h明显降低,与对照组相比,差异有统计学意义(P<0.001);照射组P53蛋白表达水平均高于对照组,在此时间点上与对照组相比,差异有统计学意义(P<0.01)。照射后4h细胞凋亡率显著升高,与对照组相比差异有统计学意义(P<0.001)。结论 survivin与P53在细胞凋亡调控中发挥重要作用,survivin表达与P53表达水平相关。 |
| 英文摘要: |
| Objective To explore the expression of survivin, an anti-apoptosis gene, in EL-4 cell line and its relationship with the expression of P53. Methods EL-4 cells were irradiated with 4 Gy X-rays to induce the expressions of survivin and P53. Flow cytometry assay was used to detect the expression of survivin and P53 and incidence of apoptosis following the irradiation. Results X-ray irradiation of 4 Gy down-regulated the expression of survivin in EL-4 cells while the expression of P53 was upregulated. A statistically lower expression of survivin was observed at 4 h after the irradiation when compared with the control group (P<0.001), while the expression of P53 was significantly increased in the exposed group (P<0.01). The incidence of apoptosis in the exposed group were significantly higher than that in the control group(P<0.001). Conclusions It is shown that survivin and P53 play important roles in regulating the cell apoptosis, and suggested that a possible correlation exists between expressions of survivin and P53. |
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