| 鞠桂芝,沈波,孙世龙,闫凤琴,付士波,李鹏武.电离辐射诱导EL-4细胞Caspase-3、P53蛋白表达及其生物学作用[J].中华放射医学与防护杂志,2006,26(6):560-562 |
| 电离辐射诱导EL-4细胞Caspase-3、P53蛋白表达及其生物学作用 |
| Expression of Caspase-3,P53 in EL-4 cells induced by ionizing radiation and its biological implications |
| 投稿时间：2006-01-10 |
| DOI： |
| 中文关键词: 电离辐射 Caspase-3 P53 凋亡 多倍体细胞 |
| 英文关键词:Ionizing radiation Caspase-3 P53 Apoptosis Polyploid |
| 基金项目:国家自然科学基金资助项目(30270346) |
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| 中文摘要: |
| 目的 研究电离辐射对EL-4细胞中Caspase-3和P53蛋白表达的影响,及其对辐射诱导细胞凋亡及多倍体细胞的作用。方法 采用单克隆抗体免疫荧光标记及流式细胞术检测蛋白表达的变化,采用PI荧光标记及流式细胞术检测细胞凋亡及多倍体细胞的变化。结果 实验表明,4.0GyX射线照射后8及12h,EL-4细胞中Caspase-3蛋白表达与对照组比较,差异有统计学意义(P<0.05);照射后2、4、8、12及24h,P53蛋白表达与对照组比较,差异有统计学意义(P<0.05或P<0.01)。实验还表明,4.0Gy照射后2、4、8、12、24、48及72h,EL-4细胞凋亡与对照组比较,差异有统计学意义(P<0.05～P<0.001);而照射后2～48h,多倍体细胞数与对照组比较则未见明显变化。结论 电离辐射可诱导EL-4细胞Caspase-3及P53蛋白表达增高,可能在辐射诱导细胞凋亡中起重要作用,而辐射诱导多倍体细胞的分子通路则可能是P53非依赖性的。 |
| 英文摘要: |
| Objective To investigate the effect of ionizing radiation on the expressions of Caspase-3 and P53 proteins in EL-4 cells and its implications in the induction of apoptosis and polyploid cells. Methods EL-4 cells were irradiated with 4.0 Gy X-rays (180 kV, 15 mA, 0.287 Gy/min). Fluorescent staining and flow cytometry analysis were used to measure protein expression, apoptosis and polyploid cells. Results It was found that the expression of Caspase-3 protein was increased significantly at 8 h and 12 h after the irradiation compared with sham-irradiated control (P<0.05), and the expression of P53 protein was also increased significantly at 2,4,8,12 and 24 h after the irradiation compared with sham-irradiated control (P< 0.05 or P<0.01). The results showed that apoptosis of EL-4 cells was increased significantly at 2,4,8,12,24,48, and 72 h after 4.0 Gy irradiation compared with sham-irradiated control (P<0.05 or P<0.01 or P<0.001). However, no significant change in the number of polyploidy cells was found during the period from 2 to 48 h after the irradiation with 4.0 Gy X-rays. Conclusions It is indicated that the expressions of Caspase-3 and P53 protein in EL-4 cells can be induced by ionizing radiation, and play an important role in the induction of apoptosis; the molecular pathway for polyploid formation might be P53-independent. |
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