| 张可杰,刘文励,付劲蓉,朱艳,黄丽芳,孙汉英,周剑峰.Notch信号在放射损伤小鼠骨髓基质细胞中的表达[J].中华放射医学与防护杂志,2006,26(6):557-559 |
| Notch信号在放射损伤小鼠骨髓基质细胞中的表达 |
| Expression of Notch in murine bone marrow stromal cells with radiation injury and its significance |
| 投稿时间：2005-12-02 |
| DOI： |
| 中文关键词: 放射损伤 骨髓基质细胞 Notch信号 |
| 英文关键词:Radiation injury Bone marrow stromal cells Notch signaling |
| 基金项目:国家自然科学基金资助项目(30570773) |
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| 摘要点击次数: 3081 |
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| 中文摘要: |
| 目的 探讨小鼠骨髓基质细胞放射损伤后Notch信号的表达。方法 应用小鼠骨髓基质细胞体外放射损伤模型,采用RT-PCR检测了小鼠骨髓基质细胞Notch1、Jagged1、Hes1基因的表达。结果 正常小鼠骨髓基质细胞中未见Notch1受体、Notch1配体Jagged1、Notch通路活化的下游基因Hes1mRNA的表达,给予60Coγ射线照射后2h即可见上述基因的表达,照射后4h达高峰,8～12h恢复正常。各组间比较,差异有统计学意义(P<0.001)。结论 γ射线作用于小鼠骨髓基质细胞后有Notch通路的活化。Notch通路参与了小鼠骨髓基质细胞的放射损伤过程。 |
| 英文摘要: |
| Objective To investigate the change of Notch expression in murine bone marrow stromal cell after exposure to γ-rays in vitro. Methods Cultured murine bone marrow stromal cells irradiated with 8.0 Gy 60Co γ-rays were used in this study. The Notch1, Jagged1, Hes1 genes expressions were analyzed by RT-PCR at 2 h, 4 h, 8 h, and 12 h after the irradiation. Results There were no expression of Notch1, Jagged1, and Hes1 genes in normal murine bone marrow stromal cells at mRNA level, while Notch1, Jagged1, and Hes1 genes were expressed at 2 h after the irradiation, and reached a peak at 4 h, then returned back to the normal level at 8-12 h. The differences between the exposed group and the control group were statistically significant (P<0.001). Conclusions Notch signaling pathway was activated in cultured murine bone marrow stromal cellls after exposure to γ-rays, it is suggested that Notch signaling pathway is involved in the radiation damage to murine bone marrow stromal cells. |
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