| 宋良文,孙丽,刁瑞英,李杨,张勇,尹纪业.巨噬细胞和MMP-9对肺成纤维细胞增生及合成Ⅳ型胶原的作用[J].中华放射医学与防护杂志,2006,26(6):549-552 |
| 巨噬细胞和MMP-9对肺成纤维细胞增生及合成Ⅳ型胶原的作用 |
| Effect of macrophage and matrix metalloproteinase-9 on proliferation of pulmonary fibroblast and synthesis of collagen Ⅳ |
| 投稿时间：2005-12-07 |
| DOI： |
| 中文关键词: 放射性肺损伤 肺泡巨噬细胞 成纤维细胞 Ⅳ型胶原 基质金属蛋白酶-9 |
| 英文关键词:Radiation pulmonary injury Alveolar macrophage Fibroblast Type Ⅳ Collagen Matrix metalloproteinase-9 |
| 基金项目:国家自然科学基金资助项目(30570545) |
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| 中文摘要: |
| 目的 探讨放射性肺纤维化早期启动的发生机制。方法 灌洗收集经60Coγ射线照射后的Wistar大鼠肺泡巨噬细胞制备条件培养液,刺激培养的人肺成纤维细胞增生,用MTT法检测细胞增生活力,Westernblot检测细胞合成Ⅳ型胶原含量的改变,酶谱分析检测细胞基质金属蛋白酶-9的活性。结果 经条件培养液刺激后,人肺成纤维细胞的增殖活力明显增加,在48～72h之内作用明显HLF合成Ⅳ型胶原增多,12h达高峰,随后有所下降;12h后基质金属蛋白酶-9活性开始增高,于48h达高峰,72h开始下降。结论 20Gy60Coγ射线能刺激AM分泌某些细胞因子促进肺间质成纤维细胞增生及合成Ⅳ型胶原,与此同时后者能激活基质金属蛋白酶-9,降解增多的ColⅣ,参与肺损伤早期的重建过程。 |
| 英文摘要: |
| Objective To explore pathogenetic mechanism in initiation of radiation-induced pulmonary fibrosis. Methods Alveolar macrophages in Wistar rats irradiated by 60Co γ-ray were collected by alveolar lavage; condition medium was prepared for stimulating human lung fibroblast (HLF) proliferation; HLF proliferation activity was determined by MTT method; collagen Ⅳ (Col Ⅳ) in HLF was determined by Western blot; the activity of matrix metalloproteinase-9 (MMP-9) was determined by zymography. Results HLF proliferation activity was significantly increased after stimulation of condition medium, and the increase was most evident within 48-72 hs. Col Ⅳ synthesis in HLF was increased and reached a peak at 12 h after stimulation and then began to decrease. MMP-9 activity began to increase at 12 h and reached a peak at 48 h and then decreased after 72 h. Conclusions Cobalt-60 gamma ray irradiation of 20 Gy can stimulate secretion of some cytokines in alveolar macrophage to promote pulmonary interstitial fibroblast proliferation and synthesis of Col Ⅳ. Col Ⅳ can stimulate MMP-9 increase; MMP-9 can degrade excess Col Ⅳ. Such changes are involved in remodeling process of early pulmonary injury. |
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