| 王志成,赵红光,杜翔,李艳博,郭伟,肖健,姚崇舜,李校堃,龚守良.莪术油对紫外线辐射皮肤损伤的防护作用[J].中华放射医学与防护杂志,2006,26(5):533-535 |
| 莪术油对紫外线辐射皮肤损伤的防护作用 |
| Protective effects of oleum curcumae wenchowensis on skin damage due to UVB |
| 投稿时间：2006-01-18 |
| DOI： |
| 中文关键词: 莪术油 UVB 抗氧化剂 防护 |
| 英文关键词:Oleum curcumae wenchowensis Ultraviolet B Antioxidant Protection |
| 基金项目:浙江省科技计划重大项目基金资助(2006-2008) |
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| 中文摘要: |
| 目的 研究莪术油对中波紫外线所致皮肤损伤的防护作用及其机制。方法 建立UVB氧化损伤模型(照射30d,累计28.38J/cm2)。观察皮肤外观及HE染色后表皮厚度和真皮成纤维细胞数量的变化。酶法检测皮肤匀浆上清液中抗氧化酶(过氧化氢酶CAT、谷胱甘肽GSH-Px和超氧化物歧化酶SOD)活性和丙二醛(MDA)含量及总抗氧化能力(T-AOC)。结果 ①光镜下,UVB照射组和基质空白组照射侧可见表皮增厚,真皮中成纤维细胞减少,而对照侧未见此病理变化;莪术油防护组照射侧表皮未见增厚,成纤维细胞数增多,但对照侧成纤维细胞数增多不明显。②UVB照射组和基质空白组照射侧皮肤中MDA含量明显升高,CAT、GSH-Px和SOD活性及T-AOC降低,而对照侧未见明显差异;莪术油防护组照射侧CAT、GSH-Px、SOD活性及T-AOC增强,MDA含量降低,而对照侧未见明显改变。结论 莪术油具有对UVB皮肤氧化损伤的防护作用,其作用机制可能与提高抗氧化酶含量及清除体内自由基有关。 |
| 英文摘要: |
| Objective To study the protective effects of oleum curcumae wenchowensis on skin damage exposed to UVB and its mechanism,and to provide the experimental basis for the protection of skin damage exposed to UVB. Methods The skin of guinea pigs was exposed to UVB (28.38 J/cm2·30 d) to establish the oxidative damage model.The skin erythema and the rough were observed during the experiment; the thickness of epiderm and the number of fibroblast were observed under light microscope after the experiment.The activities of GSH-Px,SOD,CAT and T-AOC and the contain of MDA in the supernate of skin homogenate were detected with biochemical methods. Results The epiderm in UVB exposure group and blank group thickened,but that in protective group weren't observed; the number of fibroblast in UVB exposure group and blank group decreased,while that in protective group increased,but that in control group didn't.The content of MDA in the supernate of skin homogenate in UVB exposure group and blank group increased,but that in proctective group deceased,and the activities of GSH-Px,SOD,CAT and T-AOC in UVB exposure group and blank group decreased,but those in proctective group increased,and control group had no change. Conclusions Oleum curcumae wenchowensis has the proctective effects on skin damage exposed to UVB,which may be mediated by increasing the contain of antioxidases and eliminating the free radical. |
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