中华放射医学与防护杂志

杨坤禹,胡豫,Ruebe Claudia,Ruebe Christian.肺部X射线照射后炎性细胞因子在支气管上皮细胞中的表达[J].中华放射医学与防护杂志,2006,26(5):483-485

肺部X射线照射后炎性细胞因子在支气管上皮细胞中的表达

Expressions of TNF-α, IL-1α and IL-6 in bronchiolar epithelium after thoracic irradiation

投稿时间：2006-04-30

DOI：

英文关键词:Radiation-induced lung injury Proinflammatory cytokines X rays

基金项目:

作者	单位	E-mail
杨坤禹	430023 武汉, 华中科技大学同济医学院附属协和医院肿瘤中心	huyu1964@163.net
胡豫	430023 武汉, 华中科技大学同济医学院附属协和医院血液学研究所
Ruebe Claudia	Department of Radiation Oncology, Saarland University Hospital, Hamburg/Saar 66424, Germany
Ruebe Christian	Department of Radiation Oncology, Saarland University Hospital, Hamburg/Saar 66424, Germany

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中文摘要:

目的研究C57BL/6J小鼠双肺照射12Gy后,炎性细胞因子肿瘤坏死因子-α(TNF-α)、白介素-1α(IL-1α)和白介素-6(IL-6)蛋白在肺组织中的表达。方法实验组小鼠给予双肺单次照射12Gy,对照组小鼠在相同条件下双肺假照射0Gy。分别于照射后0.5、1、3、6、12、24、48和72h,1、2、4、8、16和24周处死,通过免疫组织化学方法和图像分析定量检测肺组织中TNF-α、IL-1α和IL-6蛋白的表达。结果胸部照射后数小时,上述炎性因子蛋白表达即比对照组显著增加。在随后的急性放射性肺炎阶段,支气管上皮以及肺间质的炎性细胞产生了大量的TNF-α(最大值出现在全肺照射后第4周,9.74%±1.78%)、IL-1α(最大值出现在全肺照射后第8周,14.76%±7.77%)和IL-6(最大值出现在全肺照射后第8周,4.28%±1.33%)。结论肺部照射以后,在胸部照射的最初数小时以内,支气管上皮是上述炎性因子最重要的来源,这些炎性细胞因子进一步通过征集和激活炎症细胞来促进和放大放射性肺炎的产生和发展。

英文摘要:

Objective To investigate temporal and spatial releases of proinflam matory cytokines TNF-α, IL-1α and IL-6 in the lung tissue after thoracic irradiation in C57BL/6J mice. Methods Mice were irradiated with 12 Gy to whole lungs, and control mice were sham-irradiated. Mice were sacrificed at hours 0.5, 1, 3, 6, 12, 24, 48 and 72 and weeks 1,2, 4, 8, 16 and 24 after thoracic irradiation or sham-irradiation. Expressions of TNF-α and IL-1α, IL-6 proteins were detected with immuno-histochemistry. Results At hour 6 after thoracic irradiation, the expression of TNF-α protein increased significantly, and at hour 12 after thoracic irradiation, the expressions of IL-1α and IL-6 proteins increased significantly, too. The bronchiolar epithelium was the most prominent source of these inflammatory cytokines in the first hours. During the stage of acute pneumonitis, the bronchiolar epithelium, as well as inflammatory cells in the lung interstitium, produced high amounts of TNF-α, IL-1α and IL-6. Conclusions It was demonstrated that immediate expressions of TNF-α, IL-1α and IL-6 occurred in the bronchiolar epithelium after lung irradiation, and a long-lasting release by the bronchiolar and alveolar epithelium, and inflammatory cells during acute pneumonitis. Therefore, the bronchiolar epithelium is a significant source of proinflammatory cytokines capable of promoting inflammation through recruitment and activation of inflammatory cells after lung irradiation.

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