| 尹立杰,张力,丁田贵,彭兆祥,于环,高玉伟.甘氨双唑钠对乏氧胰腺癌细胞放射增敏研究[J].中华放射医学与防护杂志,2006,26(5):467-470 |
| 甘氨双唑钠对乏氧胰腺癌细胞放射增敏研究 |
| Radiosensitization effect of CMNa on hypoxic pancreatic cancer cell in vitro |
| 投稿时间：2005-11-23 |
| DOI： |
| 中文关键词: 胰腺癌细胞 放射增敏 细胞乏氧 甘氨双唑钠 |
| 英文关键词:Pancreatic cancer cell Radiosensitivity Cell hypoxia CMNa |
| 基金项目: |
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| 中文摘要: |
| 目的 研究甘氨双唑钠(CMNa)在乏氧环境下对人胰腺癌Panc-1细胞的放射增敏效果。方法 在乏氧和加不同浓度CMNa环境下对体外培养的人胰腺癌Panc-1细胞进行单次大剂量的γ射线照射,通过微孔板荧光酶标仪检测细胞死亡率来研究放射增敏作用。CMNa在乏氧环境下的选择性增敏效果利用流式细胞技术进行验证。结果 胰腺癌Panc-1细胞30Gy照射后的细胞死亡率在乏氧环境下随CMNa浓度的增加显著增高;而在常规环境下CMNa却无明显的放射增敏作用。CMNa在乏氧环境下的选择性增敏作用具有时间相关性。结论 CMNa在乏氧环境下对人胰腺癌细胞大剂量γ射线照射具有明显的增敏效果。 |
| 英文摘要: |
| Objective To investigate the effects of glycodidazolum natrium(CMNa) on pancreatic cancer cells under hypoxic condition. Methods The human pancreatic cancer Panc-1 cells were exposed to a single fraction of high-dose γ-ray radiation either with CMNa or under hypoxic condition. The percentage of dead cells was detected with a multiwell plated reader, and fluorescence intensities of propidium iodide were measured before and after digitonin treatment. The sensitizing effect of CMNa on cell killing induced by high-dose irradiation was evaluated by time and concentration dependence. The selective radiosensitive effect of CMNa on hypoxia was evaluated by flow cytometry. Results The death rate of pancreatic cancer Panc-1 cells paralleled with the increasing concentration of CMNa under hypoxic condition after 30 gray irradiation. The selective radiosensitive effect of CMNa on hypoxia was time-dependent. Conclusions CMNa can enhance the radiosensitivity of pancreatic cancer Panc-1 cells under hypoxic condition with high-dose irradiation. |
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