| 刘青杰,陆雪,封江彬,等.碱性单细胞凝胶电泳技术用于淋巴细胞辐射生物剂量估算初探[J].中华放射医学与防护杂志,2006,26(5):446-448.LIU Qing-jie,LU Xue,FENG Jiang-bin,et al.Preliminary study on biological dosimetry using alkaline single cell gel electrophoresis of human peripheral lymphocytes[J].Chin J Radiol Med Prot,2006,26(5):446-448 |
| 碱性单细胞凝胶电泳技术用于淋巴细胞辐射生物剂量估算初探 |
| Preliminary study on biological dosimetry using alkaline single cell gel electrophoresis of human peripheral lymphocytes |
| 投稿时间：2005-11-15 |
| DOI： |
| 中文关键词: 碱性单细胞凝胶电泳 淋巴细胞 辐射剂量估算 |
| 英文关键词:Alkaline single cell gel electrophoresis Lymphocytes Radiation dose estimation |
| 基金项目:国家自然科学基金资助项目(30570551);北京市自然科学基金资助项目(7053073) |
|
| 摘要点击次数: 3430 |
| 全文下载次数: 2072 |
| 中文摘要: |
| 目的 初步探索碱性单细胞凝胶电泳(SCGE)方法用于电离辐射生物剂量估算的可行性。方法 不同剂量水平(0～5Gy)的60Coγ射线照射正常人外周血,用碱性SCGE分析淋巴细胞“彗星”尾长和尾矩,建立各自的剂量-效应曲线。收集某基地放射工作从业人员的外周血进行碱性SCGE分析,并估算受照剂量,与监测物理剂量相比较。结果 淋巴细胞“彗星”尾长和尾矩均随着照射剂量的增加而增加,其剂量-效应曲线均为线性平方模式。以“彗星”尾长为指标的剂量-效应曲线方程为Y=13.59+20.87X-2.27X2,以尾矩为指标的曲线方程为Y=8.50+15.04X-1.43X2。参照上述方程,以尾矩为指标估算放射工作从业人员的剂量更接近实际受照剂量。结论 用碱性SCGE分析淋巴细胞“彗星”尾矩有希望成为电离辐射生物剂量计。 |
| 英文摘要: |
| Objective To explore the feasibility of alkaline single cell gel electrophoresis (SCGE) in biological dosimetry of ionizing radiation. Methods Normal peripheral blood samples from two healthy males were exposed to different doses coblat-60 gamma-rays, ranged from 0 to 5 Gy, and the tail length (TL) and Oliver tail moment (TM) of the lymphocytes were analyzed with SCGE. The dose-effect curves of TL and TM were fitted respectively. The TL and TM of lymphocytes for eight radiation workers were analyzed with SCGE, cumulative doses were estimated using the fitted TL and TM equations, and then compared with the recorded monitoring doses. Results The TLs or TMs of normal human lymphocytes were increased with the irradiation doses, and its relationship can be fitted with a linear-quadratic equations: Y=13.59+20.87X-2.27X2 for TL, and Y=8.50+15.04X-1.43X2 for TM, respectively (Y denotes TL or TM value, X is radiation dose). The doses estimated with TM equation were closer to the recorded monitoring doses than that with TL equation. Conclusions The TM in lymphocytes analyzed with SCGE is a promising radiation biological dosimeter. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|