| 姚小武,陈仕生,杨利和,等.电离辐射对血管内皮细胞纤维肌动蛋白的影响[J].中华放射医学与防护杂志,2006,26(4):363-366.YAO Xiao-wu,CHEN Shi-sheng,YANG Li-he,et al.The effect of ionizing radiation on the filamentous actin of vascular endothelial cell[J].Chin J Radiol Med Prot,2006,26(4):363-366 |
| 电离辐射对血管内皮细胞纤维肌动蛋白的影响 |
| The effect of ionizing radiation on the filamentous actin of vascular endothelial cell |
| 投稿时间：2006-01-08 |
| DOI： |
| 中文关键词: 电离辐射 血管内皮细胞 纤维肌动蛋白 |
| 英文关键词:Ionizing radiation Vascular endothelial cell Filamentous actin |
| 基金项目:广东省医学科研基金资助项目(A2003524) |
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| 中文摘要: |
| 目的 观察电离辐射对血管内皮细胞纤维肌动蛋白的影响,探讨血管内皮细胞电离辐射损伤的机制。方法 用0、2、4、6、8、10和12Gy60Coγ射线对血管内皮细胞进行照射,于照射后6h用激光共聚焦显微镜观察其细胞膜骨架蛋白,于照射后12和24h收集细胞,用流式细胞仪测定F-actin的含量。结果 细胞膜骨架随着辐射剂量的增加而破坏,这种变化呈剂量依赖性。F-actin的含量在照射后12h明显减少,24h后有所回升。结论 电离辐射能使血管内皮细胞骨架破坏和F-actin解聚,导致细胞损伤。 |
| 英文摘要: |
| Objective To observe the ionizing radiation effect on filamentous actin of vascular endothelial cell and explore its mechanism. Methods The vascular endothelial cells were irradiated with 0, 2, 4, 6, 8, 10 and 12 Gy 60Coγ -rays. The cytoskeleton was observed with CLSM at 6 hs after the irradiation and the cytoskeleton protein F-actin detected with flow cytometry after 12 and 24 hs. Results The damage to cytoskeletons increased with the radiation dose. The cytoskeleton protein F-actin was significantly decreased at 12 hs after the irradiation, and then recovered after 24 hs. Conclusion Ionizing radiation caused vascular endothelial cell injury by damaging the cytoskeleton and depolymerizating the F-actin. |
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